Proteasomal ATPases are associated with rDNA: The ubiquitin proteasome system plays a direct role in RNA polymerase I transcription
Proteasomal ATPases are associated with rDNA: The ubiquitin proteasome system plays a direct role in RNA polymerase I transcription
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DOI：
10.1016/j.bbagrm.2008.08.010
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发表时间：
2008-12-01
影响因子：
4.7
通讯作者：
Grummt, Ingrid
中科院分区：
文献类型：
--
作者：
Fatyol, Karoly;Grummt, Ingrid
在过去的几年中，已经积累了大量的数据，表明泛素蛋白酶体系统在RNA聚合酶II转录调控中的重要作用；然而，它对RNA聚合酶I转录的参与在很大程度上仍未被探索。在这项研究中，我们证明了蛋白酶体的活性是合成前rRNA所必需的。我们可以检测到蛋白酶体ATPase与rDNA启动子和编码区的关联。此外，我们还发现RNA聚合酶I相关的转录因子TIF-IA与蛋白酶体ATPase相互作用，这代表了蛋白酶体和/或蛋白酶体相关复合体被招募到rRNA基因的潜在联系。综上所述，我们的发现表明，泛素蛋白酶体系统与RNA聚合酶II系统类似，直接参与RNA聚合酶I的转录。(C)2008爱思唯尔B.V.保留所有权利。
Significant amount of data have accumulated in the last several years pointing to the essential role of the ubiquitin proteasome system in the regulation of RNA polymerase II transcription; however, its involvement in RNA polymerase I transcription has remained largely unexplored. In this study, we demonstrate that proteasome activity is required for pre-rRNA synthesis. We can detect the association of proteasomal ATPases with both the rDNA promoter and coding region. Additionally, we show that the RNA polymerase I associated transcription factor, TIF-IA interacts with proteasomal ATPases, representing a potential link via which proteasomes and/or proteasome related complexes are recruited to rRNA genes. In summary, our findings suggest that the ubiquitin proteasome system is directly involved in RNA polymerase I transcription in analogy to the RNA polymerase II system. (C) 2008 Elsevier B.V. All rights reserved.