Mass spectrometry approaches to metabolic profiling of microbial communities within the human gastrointestinal tract
Mass spectrometry approaches to metabolic profiling of microbial communities within the human gastrointestinal tract
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DOI：
10.1016/j.ymeth.2018.04.027
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发表时间：
2018-10-01
期刊：
影响因子：
4.8
通讯作者：
Takats, Zoltan
中科院分区：
文献类型：
--
作者：
Cameron, Simon J. S.;Takats, Zoltan
随着测序技术的发展，微生物群落与其环境(如人类胃肠道)之间的相互作用已成为微生物学的一个迅速发展的领域。然而，这些技术在很大程度上仅限于检测微生物群落的分类组成和/或其遗传功能能力。在这里，我们讨论了一系列基于质谱学的方法，研究人员可以使用这些方法来探索代谢水平上的宿主-微生物组相互作用。详细介绍了传统的质谱学方法，以及该领域的新发展，即环境电离质谱学和成像质谱学，我们相信这些将被证明对该领域未来的工作非常重要。我们进一步讨论了实验工作流程、数据分析选项的考虑因素，并提出了一种方法，用于建立人体胃肠道中与健康和疾病有关的功能性宿主-微生物群相互作用之间的因果关系。
The interaction between microbial communities and their environment, such as the human gastrointestinal tract, has been an area of microbiology rapidly advanced, by developments in sequencing technology. However, these techniques are largely limited to the detection of the taxonomic composition of a microbial community and/or its genetic functional capacity. Here, we discuss a range of mass spectrometry-based approaches which researchers can employ to explore the host-microbiome interactions at the metabolic level. Traditional approaches to mass spectrometry are detailed, alongside new developments in the field, namely ambient ionisation mass spectrometry and imaging mass spectrometry, which we believe will prove to be important to future work in this field. We further discuss considerations for experimental workflows, data analysis options and propose a methodology for the establishment of causal relationships between functional host-microbiome interactions with regards to health and disease in the human gastrointestinal tract.