Arabidopsis glucan synthase-like 10 functions in male gametogenesis
Arabidopsis glucan synthase-like 10 functions in male gametogenesis
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DOI：
10.1016/j.jplph.2008.06.010
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Kim, Jae-Yean
中科院分区：
文献类型：
--
作者：
Huang, Lijun;Chen, Xiong-Yan;Kim, Jae-Yean
胼胝质或 β-1,3-葡聚糖在雄性和雌性配子体发育过程中发挥多种功能。胼胝质由拟南芥中葡聚糖合酶样 (GSL) 基因家族的 12 个成员合成。为了阐明拟南芥 GSL 家族成员在性发育过程中的生物学作用，我们启动了利用 T-DNA 插入诱变系的反向遗传方法。我们筛选了 GSL 基因家族所有成员的 T-DNA 插入系，并检测了所有纯合突变幼苗。除 GSL10 之外的成员。 GSL10、gsl10-1、gsl10-3 和 gsl10-4 中的三个独立等位基因显示 T-DNA 插入片段的扭曲分离 (1:1:0)，而不是孟德斯分离 (1:2:1)。通过互交遗传分析，我们确定gsl10花粉不能遗传给后代。 GSL10/gsl10植物在四分体和小孢子阶段的突变花粉与野生型没有差异，表明GSL10对于正常小孢子生长不是必需的。对发育过程中 GSL10/gsl10 半合子花粉的分析揭示了不对称小孢子分裂的异常功能。 gsl10突变体小孢子未能进入有丝分裂。与之前描述的 GSL1、GSL2 和 GSL5 的功能不同，GSL10 涉及有丝分裂阶段花粉发育的独立过程。 (C) 2008 爱思唯尔有限公司。版权所有。
Callose or beta-1,3-glucan performs multiple functions during mate and female gametophyte development. Callose is synthesized by 12 members of the glucan synthase-like (GSL) gene family in Arabidopsis thaliana. To elucidate the biological roles of Arabidopsis GSL family members during sexual development, we initiated a reverse genetic approach with T-DNA insertional mutagenesis lines. We screened T-DNA insertion lines for all members of the GSL gene family and detected homozygous mutant seedlings for all. members except GSL10. Three independent alleles in GSL10, gsl10-1, gsl10-3 and gsl10-4 showed distorted segregation (1:1:0) of T-DNA inserts rather than Mendetian segregation (1:2:1). By genetic analysis through reciprocal cross, we determined that gsl10 pollen could not be transmitted to descendent. The mutant pollen of GSL10/gsl10 plants at tetrad and microspore stages were not different from that of wild type, suggesting that GSL10 is not essential for normal microspore growth. Analysis of GSL10/gsl10 hemizygous pollen during development revealed abnormal function in asymmetric microspore division. gsl10 mutant microspores failed to enter into mitosis. Unlike the previously described functions of GSL1, GSL2 and GSL5, GSL10 involves an independent process of pollen development at the mitotic division stage. (C) 2008 Elsevier GmbH. All rights reserved.