The oxidation of oxyhaemoglobin by glyceraldehyde and other simple monosaccharides.
The oxidation of oxyhaemoglobin by glyceraldehyde and other simple monosaccharides.
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甘油醛和其他简单单糖对氧合血红蛋白的氧化。
DOI：
10.1042/bj2170615
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Stern,A
中科院分区：
文献类型：
--
作者：
Thornalley,PJ;Wolff,SP;Crabbe,MJ;Stern,A
甘油醛和其他简单的单糖在pH 7.4和37℃的磷酸盐缓冲液中将氧合血红蛋白氧化成高铁血红蛋白，同时产生过氧化氢和单糖的α-氧代醛衍生物。在pH 7.4和37℃时，单糖还可以将高铁血红蛋白还原为高铁血红蛋白(不完整的血红蛋白)。碳氧合血红蛋白对甘油醛自氧化不起反应。用电子顺磁共振观察了单糖与血红蛋白自氧化产生的自由基。5，5-二甲基-L-吡咯啉N-氧化物自旋捕获技术。在氧合血红蛋白和高铁血红蛋白存在下，从单糖自氧化中观察到的羟基和L-羟基烷基自由基的产生被猝灭。血红蛋白似乎通过与自由基和/或自由基的烯二醇前体反应来消除自由基。
Glyceraldehyde and other simple monosaccharides oxidize oxyhaemoglobin to methaemoglobin in phosphate buffer at pH 7.4 and 37 degrees C, with the concomitant production of H2O2 and an alpha-oxo aldehyde derivative of the monosaccharide. Simple monosaccharides also reduce methaemoglobin to ferrohaemichromes (non-intact haemoglobin) at pH 7.4 and 37 degrees C. Carbonmonoxyhaemoglobin is unreactive towards oxidation by autoxidizing glyceraldehyde. Free-radical production from autoxidizing monosaccharides with haemoglobins was observed by the e.s.r. technique of spin trapping with the spin trap 5,5-dimethyl-l-pyrroline N-oxide. Hydroxyl and l-hydroxyalkyl radical production observed from monosaccharide autoxidation was quenched in the presence of oxyhaemoglobin and methaemoglobin. The haemoglobins appear to quench the free radicals by reaction with the free radicals and/or the ene-diol precursor of the free radical.