Molecular phylogeny of genus Vigna subgenus Ceratotropis based on rDNA ITS and atpB-rbcL intergenic spacer of cpDNA sequences
Molecular phylogeny of genus Vigna subgenus Ceratotropis based on rDNA ITS and atpB-rbcL intergenic spacer of cpDNA sequences
复制标题
DOI：
10.1023/a:1015158408227
复制
发表时间：
2002-02-01
期刊：
影响因子：
1.5
通讯作者：
Vaughan, DA
中科院分区：
文献类型：
--
作者：
Doi, K;Kaga, A;Vaughan, DA
利用核糖体DNA ITS区和叶绿体DNA atpB-rbcL基因间隔区序列分析了豇豆属角马亚属（Vigna subgenus Ceratotropis）种间的遗传多样性和系统发育关系。虽然两组序列的长度相似，约700 bp的rDNA-ITS是更丰富的信息比atpB-rbcL具有170%以上的多态性位点和5倍的简约信息位点。atpB-rbcL间隔区可能适用于豇豆属种以上分类群的分析。rDNA-ITS的分析结果显示，与低水平的统计偏差，分离的亚属分为三个组，对应于三个部分的Aromitifoliae，角，和Ceratotropis。根据与外群物种豇豆（Vigna unguiculata）的比较，祖先部分是Aconitifoliae。V. minima复合体、V. minima、V. riukiuensis和V. nakashimae在Angulares组内具有不同的进化路径。在角形组中的其他物种除了V. trinervia外都非常接近。三脉豇豆位于各节之间的中间位置。序列数据表明，V. reflexo-pilosa的一个基因组供体来自与V. exilis，V. hirtella和V. umbellata接近的Angulares组内的谱系。数据支持的观点，节角是最近多样化的亚属，推断短终端分支长度本节的物种之间的部分。
The genetic diversity and phylogenetic relationships among species in the genus Vigna subgenus Ceratotropis were investigated using sequence data from the ribosomal DNA ITS and atpB-rbcL intergenic spacer of chloroplast DNA regions. While both sets of sequences were of similar lengths about 700 bp the rDNA-ITS was more informative than atpB-rbcL having 170% more polymorphic sites and five times as many parsimony-informative sites. The atpB-rbcL spacer may be appropriate for analysis of taxa above the species level in the genus Vigna. Results of analyzing rDNA-ITS revealed, with low level of statistical bias, separation of the subgenus into three groups that correspond to the three sections Aconitifoliae, Angulares, and Ceratotropis. The ancestral section is Aconitifoliae based on comparison with the outgroup species cowpea, Vigna unguiculata. The V. minima complex, V. minima, V. riukiuensis, and V. nakashimae, has a distinct evolutionary path within section Angulares. Other species in section Angulares are very closely related except V. trinervia. Vigna trinervia has an intermediate position between sections. Sequence data suggests one genome donor to V. reflexo-pilosa came from a lineage within section Angulares close to V. exilis, V. hirtella, and V. umbellata. Data presented supports the view that section Angulares is the most recently diversified section in the subgenus, as inferred by short terminal branch lengths among the species of this section.