Ultrahigh molecular weight polyethylene wear debris inhibits osteoprogenitor proliferation and differentiation in vitro
Ultrahigh molecular weight polyethylene wear debris inhibits osteoprogenitor proliferation and differentiation in vitro
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DOI：
10.1002/jbm.a.32001
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发表时间：
2009-04-01
影响因子：
4.9
通讯作者：
Goodman, Stuart B.
中科院分区：
文献类型：
--
作者：
Chiu, Richard;Ma, Ting;Goodman, Stuart B.
聚乙烯磨屑通过增加骨降解和抑制骨形成来诱导进行性骨溶解。聚乙烯颗粒抑制成熟成骨细胞的功能，但聚乙烯颗粒是否也干扰成骨祖细胞的增殖和分化尚不清楚。本研究观察了超高相对分子质量聚乙烯(UHMWPE)颗粒对原代小鼠骨髓成骨祖细胞和MC3T3-E1前成骨细胞体外成骨活性的影响。通过密度梯度离心法从含血清溶液中分离出磨损模拟器试验产生的亚微米级UHMWPE颗粒。将这些颗粒以0.038、0.075、0.150、0.300和0.600%v/v的浓度涂在培养孔表面，形成一层I型胶原基质。将原代小鼠骨髓细胞和MC3T3-E1前成骨细胞接种到颗粒-胶原基质上，在成骨介质中诱导分化20d。与不含UHMWPE颗粒的对照细胞相比，UHMWPE颗粒对两种细胞的矿化、增殖、碱性磷酸酶活性和骨钙素的产生均有剂量依赖性的抑制作用。当颗粒浓度=0.150%v/v时，成骨被完全抑制。本研究表明，超高相对分子质量聚乙烯颗粒抑制成骨细胞的成骨活性，这可能导致假体周围骨的再生和修复减少。(C)2008年Wiley期刊，Inc.《生物医学杂志》89A：242-247,2009
Polyethylene wear debris induces progressive osteolysis by increasing bone degradation and suppressing bone formation. Polyethylene particles inhibit the function of mature osteoblasts, but whether polyethylene particles also interfere with the proliferation and differentiation of osteoprogenitor cells is unknown. In this study, we investigated the effects of ultrahigh molecular weight I polyethylene (UHMWPE) particles on the osteogenic activity of primary murine bone marrow osteoprogenitors and MC3T3-E1 preosteoblastic cells in vitro. Submicronsized UHMWPE particles generated from wear simulator tests were isolated from serum-containing solution by density gradient centrifugation. The particles were coated onto the surface of culture wells at concentrations of 0.038, 0.075, 0.150, 0.300, and 0.600% v/v in a layer of type I collagen matrix. Primary murine bone marrow cells and MC3T3-E1 preosteoblasts were seeded onto the particle-collagen matrix and induced to differentiate in osteogenic medium for 20 days. Exposure of both cell populations to UHMWPE particles resulted in a dose-dependent decrease in mineralization, proliferation, alkaline phosphatase activity, and osteocalcin production when compared with control cells cultured on collagen matrix without particles. Complete suppression of osteogenesis was observed at particle concentrations >= 0.150% v/v. This study demonstrated that UHMWPE particles inhibit the osteogenic activity of osteoprogenitor cells, which may result in reduced periprosthetic bone regeneration and repair. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 89A: 242-247, 2009