A nanoprobe for fluorescent monitoring of microRNA and targeted delivery of drugs.
A nanoprobe for fluorescent monitoring of microRNA and targeted delivery of drugs.
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DOI：
10.1039/d1ra00154j
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发表时间：
2021-02-23
期刊：
影响因子：
3.9
通讯作者：
Xie G
中科院分区：
文献类型：
--
作者：
Zuo C;Guo Y;Li J;Peng Z;Bai S;Yang S;Wang D;Chen H;Xie G
可用于监测特定生物标志物的表达并用作受控药物递送的载体的多功能纳米材料是非常期望的。在本文中，我们报道了一种新的DNA混合门控核壳上转换纳米探针（UCNP@MOF/DOX），用于microRNA-21（miR-21）的荧光分析，这也触发了探针中负载的药物的释放，用于按需抗癌治疗。该纳米探针是基于近红外（NIR）激发的上转换纳米颗粒（UCNPs）的紫外-可见光和金属有机框架（MOFs）用于药物递送的非凡负载能力的优点而构建的。通过miR-21从纳米探针中控制释放多柔比星（DOX）经历了以下两个阶段的动力学：由miR-21特异性触发并与miR-21浓度成比例的快速释放阶段，以及在门控和非门控纳米探针中观察到的缓慢阶段，这是由于UIO-66-NH 2涂层通过与磷酸盐的配体交换而塌陷。此外，纳米探针显示出良好的选择性，对miR-21的线性响应范围为4 nM至500 nM，检测限为4 nM，这排除了由于正常细胞中低丰度内源性miR-21表达而导致的非预期有效负载泄漏。此外，基于由AS 1411介导的DOX识别和响应性释放构成的双靶向递送系统，在MCF-7细胞中观察到特异性细胞毒性功效。目前的工作提供了一种智能和强大的纳米探针，用于实时检测肿瘤细胞中的miRNA和双响应药物递送。制备用于miR-21的荧光监测和DOX的按需递送两者的DNA混合门控核-壳上转换纳米探针。它对miR-21显示出良好的选择性，并对MCF-7细胞表现出特异性细胞毒性功效。
Multifunctional nano-materials that can be used to monitor the expression of specific biomarkers and serve as vehicles for controlled drug delivery are highly desirable. Herein, we report a new DNA-hybrid-gated core–shell upconversion nanoprobe (UCNP@MOF/DOX) for fluorescence analysis of microRNA-21 (miR-21), which also triggers the release of drug loaded in the probes for on-demand anti-cancer treatment. The nanoprobe is built on the merits of ultraviolet-visible light of upconversion nanoparticles (UCNPs) excited by near-infrared (NIR) and extraordinary loading capability of metal–organic frameworks (MOFs) for drug delivery. Controlled release of doxorubicin (DOX) from the nanoprobe by miR-21 underwent the following two-stage kinetics: a fast release stage specifically triggered by miR-21 and proportional to miR-21 concentration and a slow stage observed in both gated and ungated nanoprobes due to collapse of the UIO-66-NH2 coatings via ligand exchange with phosphates. In addition, the nanoprobe showed good selectivity, a linear response towards miR-21 ranging from 4 nM to 500 nM, and a limit of detection in 4 nM, which precluded unintended payload leakage due to low-abundance endogenous miR-21 expression in normal cells. Moreover, based on a dual-targeted delivery system constituted by AS1411-mediated recognition and responsive release of DOX, a specific cytotoxic efficacy was observed in MCF-7 cells. The present work provides a smart and robust nanoprobe for real-time detection of miRNA and dual-responsive drug delivery in tumor cells. A DNA-hybrid-gated core–shell upconversion nanoprobe is prepared for both fluorescent monitoring of miR-21 and on-demand delivery of DOX. It showed good selectivity towards miR-21 and demonstrated specific cytotoxic efficacy towards MCF-7 cells.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
14
作者：
Ji, Yu;Lu, Feng;Huang, Wei
通讯作者：
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影响因子：
3.9
作者：
Vazquez-Gonzalez, Margarita;Willner, Itamar
通讯作者：
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影响因子：
8.4
作者：
Xu J;Han W;Cheng Z;Yang P;Bi H;Yang D;Niu N;He F;Gai S;Lin J
通讯作者：
Lin J
影响因子：
3
作者：
Guo, Chenyi;Hu, Peng;Yuan, Fangli
通讯作者：
Yuan, Fangli