Use of synchrotron radiation microangiography to assess development of small collateral arteries in a rat model of hindlimb ischemia.
Use of synchrotron radiation microangiography to assess development of small collateral arteries in a rat model of hindlimb ischemia.
复制标题
使用同步辐射显微血管造影评估大鼠后肢缺血模型中小侧支动脉的发育。
DOI：
10.1161/01.cir.95.4.805
复制
发表时间：
1997
期刊：
影响因子：
37.8
通讯作者：
Hideo Miyashita
中科院分区：
文献类型：
--
作者：
Satoshi Takeshita;Takaaki Isshiki;Hidezo Mori;E. Tanaka;Koji Eto;Y. Miyazawa;A. Tanaka;Y. Shinozaki;Kazuyuki Hyodo;Masami Ando;M. Kubota;Kenkichi Tanioka;Keiji Umetani;Masahiko Ochiai;Tomohide Sato;Hideo Miyashita
背景
目前的血管造影方法不能提供测量直径< 200微米的动脉的图像。我们最近开发了一种新的血管造影系统，它使用单色同步辐射和空间分辨率为30微米的高清视频系统。在本研究中，我们应用这种微血管造影系统，以显示正常和缺血大鼠肢体的小动脉，并调查侧支动脉的发展。
方法和结果
切除股动脉后4周，对正常和缺血肢体进行微血管造影。在正常肢体中，髂动脉和/或股动脉的第四分支（直径< 100微米）很容易识别。发现其中一些分支灌注了大腿远端区域。在缺血肢体中，观察到血管网络的广泛结构重塑。髂动脉的分支发育出许多小动脉，构成了一个精细的动脉网络，即所谓的“中间区”，由线性的、正常外观的动脉和具有起伏的、不分支外观的动脉组成。
结论
血管造影显示小的侧支动脉网络，分辨率极限< 100微米。线性侧支循环似乎是由预先存在的血管开放引起的。在正常肢体中未观察到波状的、不分支的血管，并且似乎是肢体缺血后新形成的血管。同步辐射微血管造影似乎是一个强大的手段，评估小侧支动脉的发展，这可能有助于提供一个基础，了解侧支循环。
BACKGROUND
Current methods of angiography cannot provide images of arteries measuring < 200 microns in diameter. We have recently developed a new angiography system that uses monochromatic synchrotron radiation and a high-definition video system with a spatial resolution of 30 microns. In the present study, we applied this microangiography system to visualize small arteries in normal and ischemic rat limbs and investigated the development of collateral arteries.
METHODS AND RESULTS
Microangiography was performed in the normal and the ischemic limb 4 weeks after the excision of the femoral artery. In the normal limb, up to the fourth branches of the iliac and/or femoral arteries (diameter < 100 microns) were readily identified. Some of these branches were found to perfuse the distal thigh area. In the ischemic limb, an extensive structural remodeling of the vascular network was observed. Numerous small arteries had developed from the branches of the iliac artery to constitute a fine arterial network, the so-called "midzone," which was composed of linear, normal appearing arteries and those with an undulating, unbranched appearance.
CONCLUSIONS
The small collateral artery network was angiographically visualized with a resolution limit < 100 microns. The linear collaterals appeared to result from an opening of preexisting vessels. The undulating, unbranched vessels were not observed in the normal limbs and seemed to be vessels that were newly formed after limb ischemia. Synchrotron radiation microangiography appears to be a powerful means of assessing the development of small collateral arteries, which may help to provide a basis for understanding of the collateral circulation.
影响因子：
15.9
作者：
TAKESHITA, S;ZHENG, LP;ISNER, JM
通讯作者：
ISNER, JM
通过 5-羟色胺-2 受体特异性增加犬后肢侧支动脉树对血清素的敏感性。
影响因子：
3.1
作者：
Orlandi,C;Blackshear,JL;Hollenberg,NK
通讯作者：
Hollenberg,NK