MnO2 Nanosheet-Carbon Dots Sensing Platform for Sensitive Detection of Organophosphorus Pesticides.
MnO2 Nanosheet-Carbon Dots Sensing Platform for Sensitive Detection of Organophosphorus Pesticides.
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DOI：
10.1021/acs.analchem.7b04193
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发表时间：
2017-12
影响因子：
7.4
通讯作者：
Xu Yan;Yang Song;Chengzhou Zhu;Hongxia Li;Dan Du;X. Su;Yuehe Lin
中科院分区：
文献类型：
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作者：
Xu Yan;Yang Song;Chengzhou Zhu;Hongxia Li;Dan Du;X. Su;Yuehe Lin
碳点（CDs）与纳米材料淬灭剂的结合为设计有前途的传感器创造了一种创新的方法。本文设计了一种新型荧光传感平台，用于有机磷农药（OPs）的灵敏检测。采用3-氨基苯硼酸一步水热反应法制备CDs。二氧化锰（MnO2）纳米片可以通过Förster共振能量转移（FRET）猝灭CDs的荧光。在丁基胆碱酯酶（BChE）和乙酰硫代胆碱存在下，酶解产物（硫代胆碱）可以有效地触发MnO2纳米片的分解，从而恢复CDs荧光。OPs作为BChE活性抑制剂可以阻止硫代胆碱的生成和MnO2纳米片的分解，伴随着系统的荧光“关闭”。因此，BChE-ATCh-MnO2-CDs体系可以基于荧光开关的“开-关”来定量检测OPs。在最佳条件下，基于fret的方法可以检测0.05 ~ 5 ng mL-1的对氧磷，检出限为0.015 ng mL-1。同时，该策略还显示出一种浓度依赖性的视觉颜色变化。因此，该方法有可能成为OPs检测的候选方法。
Carbon dots (CDs) combined with a nanomaterial-based quencher has created an innovative way for designing promising sensors. Herein, a novel fluorescent-sensing platform was designed for sensitive detection of organophosphorus pesticides (OPs). The preparation of CDs was based on one-step hydrothermal reaction of 3-aminobenzeneboronic acid. The fluorescence of CDs can be quenched by manganese dioxide (MnO2) nanosheets via the Förster resonance energy transfer (FRET). In the presence of butyrylcholinesterase (BChE) and acetylthiocholine, the enzymatic hydrolysate (thiocholine) can efficiently trigger the decomposition of MnO2 nanosheets, resulting in the recovery of CDs fluorescence. OPs as inhibitors for BChE activity can prevent the generation of thiocholine and decomposition of MnO2 nanosheets, accompanying the fluorescence "turn-off" of the system. So the BChE-ATCh-MnO2-CDs system can be utilized to detect OPs quantitatively based on the fluorescence turn "on-off". Under the optimum conditions, the present FRET-based approach can detect paraoxon ranging from 0.05 to 5 ng mL-1 with a detection limit of 0.015 ng mL-1. Meanwhile, the present strategy also showed a visual color change in a concentration-dependent manner. Thus, the proposed assay can potentially be a candidate for OPs detection.