LYMPHOCYTE ADHESION TO BRAIN CAPILLARY ENDOTHELIAL-CELLS IN-VITRO
LYMPHOCYTE ADHESION TO BRAIN CAPILLARY ENDOTHELIAL-CELLS IN-VITRO
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DOI：
10.1016/0165-5728(94)90155-4
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发表时间：
1994-06-01
影响因子：
3.3
通讯作者：
KUIPER, J
中科院分区：
文献类型：
--
作者：
DEVRIES, HE;MOOR, ACE;KUIPER, J
研究了牛脑内皮细胞（BBEC）上粘附分子的存在和上调。将BBEC单层与脂多糖（LPS）、白细胞介素-1 β（rhIL-1 β）和白细胞介素-6（rhIL-6）一起孵育，以在体外模拟脑毛细血管中的炎症部位。用5或10 ng/ml LPS刺激内皮细胞4 h后，淋巴细胞与BBEC的粘附增加4.1倍。淋巴细胞粘附增加后的BBEC与IL-1孵育4小时，并增加了3.7倍，使用100 ng/ml IL-1。用IL-6预孵育4 h的BBEC也显示淋巴细胞粘附增加，用100 ng/ml IL-6预处理的细胞显示淋巴细胞粘附增加3倍。针对CD 11 a、CD 18和VLA-4的特异性单克隆抗体能够阻断淋巴细胞与刺激的BBEC的粘附。这些结果表明，在体外激活的BBEC可以作为一个模型，用于研究炎症的血脑屏障。
The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1 beta (rhIL-1 beta), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier.