Determination of DMPC hydration in the L(alpha) and L(beta') phases by H-2 solid state NMR of D2O

Determination of DMPC hydration in the L(alpha) and L(beta') phases by H-2 solid state NMR of D2O
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DOI:
10.1016/s0014-5793(97)00201-9
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发表时间:
1997-04-01
期刊:
影响因子:
3.5
通讯作者:
Dufourc, EJ
Dufourc, EJ
中科院分区:
生物学3区
文献类型:
--
作者:
Faure, C;Bonakdar, L;Dufourc, EJ

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用D_2O的固体氘核磁共振研究了在流体(L(α))和凝胶(L(β ′))相中与二肉豆蔻酰磷脂酰胆碱(DMPC)界面结合的水分子数。我们确定每个DMPC分子在流体和凝胶相中分别结合9.7 +/- 0.5和小于4.3 +/- 0.5 D2 O。这些结果是占考虑DMPC结合位点的数量,以及在每个阶段的分子组织。(C)1997年欧洲生物化学学会联合会。
The number of water molecules bound to the dimyristoylphosphatidylcholine (DMPC) interface was investigated both in the fluid (L(alpha)) and gel (L(beta')) phases by solid state deuterium NMR of D2O. We determined that each DMPC molecule binds 9.7 +/- 0.5 and less than 4.3 +/- 0.5 D2O in the fluid and gel phases respectively. These results are accounted for by considering the number of DMPC binding sites as well as the molecular organization in each phase. (C) 1997 Federation of European Biochemical Societies.