Cellular responses and cytokine profiles in Ascaris lumbricoides and Trichuris trichiura infected patients
Cellular responses and cytokine profiles in Ascaris lumbricoides and Trichuris trichiura infected patients
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DOI：
10.1046/j.1365-3024.2002.00600.x
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发表时间：
2002-11-01
影响因子：
2.2
通讯作者：
Corrêa-Oliveira, R
中科院分区：
文献类型：
--
作者：
Geiger, SM;Massara, CL;Corrêa-Oliveira, R
在年轻人中研究了肠道蠕虫感染（即蛔虫和鞭虫）对细胞反应性和细胞因子产生的影响。在无寄生虫的地方性对照中，蛔虫特异性细胞反应性高于感染毛毛虫或蛔虫的患者或同时感染这两种寄生虫的患者。此外，阴性地方病对照中外周血单核细胞（PBMC）分泌的有丝分裂原诱导的肿瘤坏死因子（TNF）-α、白细胞介素（IL）-12和干扰素（IFN）-γ分泌量高于感染个体。在单独蛔虫以及共同感染的患者中，蛔虫抗原特异性产生的 TNF-α、IL-12 和 IFN-γ 较低，而 IL-10 和 IL-13 的分泌则升高，并且在所有患者组中同样较高。鞭虫特异性和蛔虫特异性 IgG4 的检测显示，与流行病对照相比，鞭虫或蛔虫患者的血清抗体水平显着较高（P < 0.05），而感染个体和流行病对照中寄生虫特异性 IgE 抗体水平同样较高。总之，与地方性对照相比，寄生虫负荷较高的慢性感染蛔虫和鞭虫患者的细胞反应性降低，1 型 TNF-α、IFN-γ 和 IL-12 反应较低，而流行区所有组中 2 型 IL-10 和 IL-13 的产生相似。前者可能支持寄生虫的持续存在，而大量的2型细胞因子释放可能促进保护性免疫，这表明宿主进行适应以控制寄生虫负担，同时最大限度地减少免疫介导的宿主自身损伤。
The impact of intestinal helminth infection, i.e. Ascaris lumbricoides and Trichuris trichiura, on cellular responsiveness and cytokine production was investigated in young adults. Ascaris-specific cellular responsiveness was higher in parasite-free endemic controls than in patients infected with T. trichiura, or A. lumbricoides, or patients co-infected with both parasites. Also, mitogen-induced tumour necrosis factor (TNF)-alpha, interleukin (IL)-12 and interferon (IFN)-gamma secretion by peripheral blood mononuclear cells (PBMC) was higher in negative endemic controls than in infected individuals. Ascaris antigen-specific production of TNF-alpha, IL-12 and IFN-gamma was low in singly Ascaris as well as in co-infected patients, whereas secretion of IL-10 and IL-13 was elevated and similarly high in all patient groups. The detection of Trichuris-specific and Ascaris-specific IgG4 revealed significantly higher serum antibody levels in Trichuris or Ascaris patients when compared to endemic controls (P < 0.05), whereas parasite-specific IgE antibody levels were similarly high in infected individuals and in endemic controls. In summary, chronically infected Ascaris and Trichuris patients with a high parasite load presented reduced cellular reactivity and lower type 1 TNF-α, IFN-γ and IL-12 responses when compared with endemic controls, whereas type 2 IL-10 and IL-13 productions were similar in all groups from the endemic area. The former may support parasite persistence, whereas substantial type 2 cytokine release may promote protective immunity, suggesting an adaptation of the host to control the parasite burden while minimizing immune-mediated host self-damage.