Slx5p-Slx8p Promotes Accurate Chromosome Segregation by Mediating the Degradation of Synaptonemal Complex Components during Meiosis
Slx5p-Slx8p Promotes Accurate Chromosome Segregation by Mediating the Degradation of Synaptonemal Complex Components during Meiosis
复制标题
Slx5p-Slx8p 通过介导减数分裂过程中联会复合体成分的降解来促进染色体的准确分离
DOI：
10.1002/advs.201900739
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发表时间：
2020-01-01
期刊：
影响因子：
15.1
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
Liu, Chao;Zhao, Haichao;Li, Wei
减数分裂增加了遗传多样性，但基因组补体需要稳定以确保后代的生存能力。小泛素样修饰子（small ubiquitin-like modifier， SUMO）和泛素都参与了减数分裂的调控，但SUMO-泛素化串扰在减数分裂中的作用尚不清楚。在这里，据报道，sumo靶向的泛素连接酶Slx8p在减数分裂期间促进准确的染色体分离，因为SLX8的缺失导致突触复合体（SC）成分降解缺陷导致非整倍体增加。Slx8p的RING结构域和SUMO相互作用基序都是减数分裂过程和维持孢子活力所必需的，并且在slx8缺失菌株中，与SUMO融合的tetraubiquitin的表达部分地挽救了减数分裂缺陷。Slx5p-Slx8p可以直接将泛素添加到SUMOylated的Zip1p和Ecm11p上，Ecm11p的强制降解部分修复了SLX8缺失菌株的产孢缺陷。这些发现提供了SC拆卸的机制，并揭示了SUMOylation和泛素化之间的串扰通过促进减数分裂过程中SC成分的降解来促进准确的染色体分离。
Meiosis increases genetic diversity, yet the genome complement needs to be stable to ensure offspring viability. Both small ubiquitin-like modifier (SUMO) and ubiquitin have been reported to participate in meiotic regulation, yet functions of the SUMO-ubiquitination crosstalk in meiosis remain unclear. Here, it is reported that a SUMO-targeted ubiquitin ligase, Slx8p, promotes accurate chromosome segregation during meiosis, since the deletion of SLX8 leads to increased aneuploidy due to a defect in synaptonemal complex (SC) component degradation. Both the RING domain and SUMO interacting motifs of Slx8p are essential for meiotic progression and maintaining spore viability, and the expression of tetraubiquitin fused with SUMO partially rescues meiotic defects in the SLX8-deletion strain. Furthermore, Slx5p-Slx8p can directly add ubiquitin to SUMOylated Zip1p and Ecm11p, and forced degradation of Ecm11p partially rescues the sporulation defects of the SLX8 deletion strain. These findings provide a mechanism for SC disassembly and reveal that the crosstalk between SUMOylation and ubiquitination facilitates accurate chromosome segregation by promoting SC component degradation during meiosis.