In vivo femtosecond laser-assisted posterior lamellar keratoplasty in rabbits.
In vivo femtosecond laser-assisted posterior lamellar keratoplasty in rabbits.
复制标题
兔体内飞秒激光辅助后板层角膜移植术。
DOI：
10.1097/01.ico.0000231491.95377.0b
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发表时间：
2006
期刊：
影响因子：
2.8
通讯作者：
Juhasz,Tibor
中科院分区：
文献类型：
--
作者：
Mian,ShahzadI;Soong,HKaz;Patel,SanjayV;Ignacio,Teresa;Juhasz,Tibor
目的：建立飞秒激光辅助兔后板层角膜移植术模型。方法：采用飞秒激光对11只家兔12眼进行角膜后板层界面及圆环（侧）切口。激光参数为：能量6.0 ~ 8.7 μJ（片层切割）和6.0 ~ 8.8 μJ（环钻切割），光斑尺寸2.4 μm，射速15 kHz，环钻直径6.0 ~ 7.0 mm。在所有的眼睛中，通过角膜周围的刀片切口激光治疗后，将角膜后盘从眼睛中取出。将同一切除的角膜盘重新定位到后基质床，模拟后板层移植。3只兔术后立即去核4眼，8只兔术后平均随访17.9±6.5周去核8眼。采用光镜和扫描电镜观察角膜切面。结果：飞秒激光在兔眼后板层切口及环钻切口的形成均取得了成功。角膜后盘厚度为204.3±21 μm（占角膜中央厚度的56.9%），术后角膜屈光度为49.1±5.8 d， 3眼出现符合角膜扩张的临床表现。结论：飞秒激光对兔眼后板层角膜移植术的非机械性切口相对容易、可靠。为了防止角膜扩张，可能需要维持最小的角膜前厚度。
Purpose:To develop a rabbit model for femtosecond laser-assisted posterior lamellar keratoplasty.Methods:The femtosecond laser was used to make the posterior corneal lamellar interface and trephine (side) cut in 12 eyes of 11 rabbits. Laser parameters were energy 6.0 to 8.7 (lamellar cut) and 6.0 to 8.8 μJ (trephination cut), spot size 2.4 μm, firing rate 15 kHz, and trephination diameter 6.0 to 7.0 mm. In all eyes, the posterior corneal disc was removed from the eye after laser treatment through a blade incision in the peripheral cornea. The same excised corneal disc was repositioned into the posterior stromal bed to simulate posterior lamellar transplantation. Four eyes of 3 rabbits were enucleated immediately after surgery, and 8 eyes of 8 rabbits were enucleated after a mean follow-up of 17.9±6.5 weeks. The corneal cut surfaces were examined by light microscopy and scanning electron microscopy.Results:The femtosecond laser was successful in producing posterior lamellar and trephination cuts in rabbit eyes. The thickness of the posterior corneal discs was 204.3±21 μm (56.9% of central corneal thickness), and postoperative keratometry was 49.1±5.8 D. Clinical appearance consistent with corneal ectasia was noted in 3 eyes.Conclusion:The femtosecond laser can make nonmechanical cuts for posterior lamellar keratoplasty with relative ease and reliability in rabbit eyes. A minimum residual anterior corneal thickness may need to be maintained to prevent ectasia.