Creating ultrathin nanoscopic collagen matrices for biological and biotechnological applications
Creating ultrathin nanoscopic collagen matrices for biological and biotechnological applications
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DOI：
10.1002/smll.200600598
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发表时间：
2007-06-01
期刊：
影响因子：
13.3
通讯作者：
Muller, Daniel J.
中科院分区：
文献类型：
--
作者：
Cisneros, David A.;Friedrichs, Jens;Muller, Daniel J.
材料的生物功能化产生了蛋白质、细胞或组织可以完成天然或期望任务的界面。在这里，我们报告了如何控制组装的I型胶原蛋白到定义明确的纳米矩阵的不同模式。在这些纳米（约3 nm）基质中的胶原原纤维保持其在体内观察到的天然结构。这开辟了使用胶原结合蛋白或与胶原结合结构域融合的蛋白质来创建可编程生物功能化基质的可能性。应用于真核细胞，这些纳米结构的基质可以指导细胞过程，如粘附，定向和迁移。
The biofunctionalization of materials creates interfaces on which proteins, cells, or tissues can fulfill native or desired tasks. Here we report how to control the assembly of type I collagen into well-defined nanoscopic matrices of different patterns. Collagen fibrils in these ultrathin (approximate to 3 nm) matrices maintained their native structure as observed in vivo. This opens up the possibility to create programmable biofunctionalized matrices using collagen-binding proteins or proteins fused with collagen-binding domains. Applied to eukaryotic cells, these nanostructured matrices can direct cellular processes such as adhesion, orientation and migration.