An initial characterization of the serum phosphoproteome.
An initial characterization of the serum phosphoproteome.
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DOI：
10.1021/pr900603n
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发表时间：
2009-12
影响因子：
4.4
通讯作者：
Petricoin, Emanuel F., III
中科院分区：
文献类型：
--
作者：
Zhou, Weidong;Ross, Mark M.;Tessitore, Alessandra;Ornstein, David;VanMeter, Amy;Liotta, Lance A.;Petricoin, Emanuel F., III
磷酸化是一种动态的翻译后蛋白质修饰，是维持和调节蛋白质结构和功能的一般机制的基础，当然也是通过信号转导支持关键细胞过程。在过去的几年中，许多研究的大规模分析磷蛋白和映射磷酸化位点从培养的人类细胞或组织的质谱技术已经出版，但是，有很少的信息一般（或全球）磷蛋白质组学表征和描述的磷蛋白分析物的内容内循环。循环磷蛋白和磷酸肽可以代表重要的疾病生物标志物，因为它们在细胞功能中的重要性是众所周知的，并且这些分析物在人类疾病如癌症中经常突变和活化。在这里，我们报告了一个初步的尝试，以表征磷蛋白含量的血清。为了实现这一点，我们开发了一种方法，其中从消化的血清蛋白中富集磷酸肽，并使用LTQ-Orbitrap（CID）和LTQ-ETD质谱仪通过LC-MS/MS进行分析。使用这种方法，我们确定了约100个独特的磷酸肽与严格的过滤标准和低于1%的错误发现率。
Phosphorylation is a dynamic post-translational protein modification that is the basis of a general mechanism for maintaining and regulating protein structure and function, and of course underpins key cellular processes through signal transduction. In the last several years, many studies of large-scale profiling of phosphoproteins and mapping phosphorylation sites from cultured human cells or tissues by mass spectrometry technique have been published; however, there is little information on general (or global) phosphoproteomic characterization and description of the content of phosphoprotein analytes within the circulation. Circulating phosphoproteins and phosphopeptides could represent important disease biomarkers because of their well-known importance in cellular function, and these analytes frequently are mutated and activated in human diseases such as cancer. Here we report an initial attempt to characterize the phosphoprotein content of serum. To accomplish this, we developed a method in which phosphopeptides are enriched from digested serum proteins and analyzed by LC-MS/MS using LTQ-Orbitrap (CID) and LTQ-ETD mass spectrometers. Using this approach we identified ~100 unique phosphopeptides with stringent filtering criteria and a lower than 1% false discovery rate.
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DOI：
10.2174/1568016052773351
发表时间：
2005-01-05
期刊：
Current Medicinal Chemistry - Cardiovascular & Hematological Agents
影响因子：
--
作者：
Marcondes, S.;Antunes, E.
通讯作者：
Antunes, E.
DOI：
10.1073/pnas.0607084104
发表时间：
2007-02-13
影响因子：
11.1
作者：
Chi, An;Huttenhower, Curtis;Hunt, Donald F.
通讯作者：
Hunt, Donald F.
影响因子：
3.4
作者：
Pan, Cuiping;Gnad, Florian;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
3.4
作者：
Deutsch, EW;Eng, JK;Aebersold, R
通讯作者：
Aebersold, R
影响因子：
7
作者：
Macek, Boris;Gnad, Florian;Mann, Matthias
通讯作者：
Mann, Matthias