The Dynamics of Gene Products Fluctuation During Bovine Pre-Hatching Development
The Dynamics of Gene Products Fluctuation During Bovine Pre-Hatching Development
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DOI：
10.1002/mrd.21030
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发表时间：
2009-08-01
影响因子：
2.5
通讯作者：
Robert, Claude
中科院分区：
文献类型：
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作者：
Gilbert, Isabelle;Scantland, Sara;Robert, Claude
早期胚胎发育，从受精到胚泡孵化，是一个非常动态的发育窗口，其特征在于，特别是在大型哺乳动物中，在母体到胚胎过渡（MET）期间结束的转录无功能期。在MET之前，第一个细胞周期由卵子发生期间积累的储存的RNA和蛋白质池支持。因此，RNA和蛋白质含量在发育阶段之间是不同的。还已知储存的mRNA的稳定性和翻译募集的机制部分地由poly（A）尾的长度控制。到目前为止，很少有人知道RNA和蛋白质含量的波动在孵化前的时期。在这份报告中，我们提出的测量总RNA，mRNA，聚（A）轴承mRNA和蛋白质含量，以及估计的比例，这两个mRNA馏分总RNA含量在这些发展阶段。我们发现，虽然不同的转录本内容的个体发育概况是预期的，但它们的量大大低于报道的值。此外，低28S rRNA丰度和MET前蛋白质含量减少的趋势表明核糖体周转和翻译的潜力有限。我们认为RNA和蛋白质含量的总体波动是跨阶段基因表达数据的下游解释所必需的参考点，无论是通过候选方法还是高通量方法。
Early embryonic development, spanning fertilization to blastocyst hatching, is a very dynamic developmental window that is characterized, especially in large mammals, by a period of transcriptional incompetence that ends during the maternal to embryonic transition (MET). Prior to the MET the first cell cycles are supported by stored RNA and proteins pools accumulated during oogenesis. Therefore, RNA and protein content are different between developmental stages. It is also known that the stability of the stored mRNA and the mechanisms for translation recruitment are partly controlled by the length of the poly(A) tail. To date, little is known about RNA and protein content fluctuations during the pre-hatching period. In this report we present measurements of total RNA, mRNA, poly(A) bearing mRNA and protein contents, as well as estimations of the proportions of both mRNA fractions to total RNA contents within these developmental stages. We found that while the ontogenic profiles of the different transcript contents were expected, their amounts were considerably lower than the reported values. Additionally, low 28S rRNA abundance and a tendency for diminishing protein content prior to the MET, suggest a limited potential for ribosomal turnover and translation. We consider the overall fluctuations in RNA and protein contents to be reference points that are essential for downstream interpretation of gene expression data across stages whether it be through candidates or high throughput approaches.