In Vitro and In Vivo Evaluation of Tetracycline Loaded Chitosan-Gelatin Nanosphere Coatings for Titanium Surface Functionalization
In Vitro and In Vivo Evaluation of Tetracycline Loaded Chitosan-Gelatin Nanosphere Coatings for Titanium Surface Functionalization
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用于钛表面功能化的四环素负载壳聚糖-明胶纳米球涂层的体外和体内评价
DOI：
10.1002/mabi.201600130
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发表时间：
2017-02-01
影响因子：
4.6
通讯作者：
Jiang, Tao
中科院分区：
文献类型：
--
作者：
Ma, Kena;Cai, Xinjie;Jiang, Tao
由于钛表面的生物相容性，钛植入物是微生物定植和生物膜形成的合适基质，这仍然是一个严重的临床威胁。目前的研究方向主要集中在钛基体或抗粘附表面的抗菌涂层的开发上。在我们以前的研究中，四环素（Tc）负载壳聚糖-明胶（CSG）纳米球涂层成功地制备钛基板上通过电泳沉积。这些涂层具有纳米球结构，并具有优异的体外抗菌性能。然而，在未来的应用中，需要对涂层进行进一步的体外和体内评价。因此，在本研究中，作者研究了涂层的机械性能，溶胀和降解性能，体外细胞反应的前成骨细胞，和抗菌性能在兔。结果表明，Tc的加入提高了涂层的拉伸结合强度，降低了涂层的溶胀率，加速了涂层的降解。尽管高Tc浓度组对MC 3 T3-E1细胞表现出细胞毒性，但其体内抗菌性是优选的，并且显示出比预防性施用Tc更好的结果。Tc负载CSG纳米球涂层是用于钛表面功能化的合适的抗菌涂层。
Owing to the biocompatibility of titanium surface, titanium implants are suitable substrates for microbial colonization and biofilm formation, which is still a serious clinical threat. Current research trends have been focused on the development of antibacterial coatings on titanium substrate or adhesion resistant surface. In our previous study, tetracycline (Tc) loaded chitosan-gelatin (CSG) nanosphere coatings are successfully fabricated on titanium substrates via electrophoretic deposition. These coatings show nanosphere structure, and excellent antibacterial property in vitro. However, further in vitro and in vivo evaluation of the coatings is required for the future application. Therefore, in the present study, the authors investigate the coatings' mechanical, swelling and degradation property, in vitro cellular response to preosteoblast cells, and the antibacterial property in rabbits. Results show that Tc incorporation can improve the tensile bond strength of the coating, decrease the swelling ratio, and accelerate the degradation of the coating. Although high Tc concentration group exhibits cytotoxicity to MC3T3-E1 cells, its in vivo antibacterial property is preferred, and shows better outcome than the prophylactic administration of Tc. Tc loaded CSG nanosphere coatings are suitable antibacterial coatings for titanium surface functionalization.