Conditional knockout of transient receptor potential melastatin 7 in the enamel epithelium: Effects on enamel formation.
Conditional knockout of transient receptor potential melastatin 7 in the enamel epithelium: Effects on enamel formation.
复制标题
有条件地敲除牙釉质上皮细胞中瞬时受体电位 melastatin 7：对牙釉质形成的影响。
DOI：
10.1111/eos.12920
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发表时间：
2023
影响因子：
1.9
通讯作者：
Okabe K.
中科院分区：
文献类型：
--
作者：
Shin M;Matsushima A;Kajiya H;Okamoto F;Ogata K;Oka K;Ohshima H;Bartlett JD;Okabe K.
瞬时受体电位melastatin 7（TRPM 7）是一种连接到激酶结构域的独特离子通道。我们先前证明了Trpm 7在小鼠成釉细胞和成牙本质细胞中表达高，并且在TRPM 7激酶死亡的小鼠中釉质形成受损。在此，我们分析了角蛋白14-Cre; Trpm 7 fl/fl条件性敲除（cKO）小鼠和Trpm 7敲低细胞系中TRPM 7在釉质形成过程中的功能。cKO小鼠显示出比对照小鼠更少的牙齿色素沉着和断裂的切牙尖端。cKO小鼠牙釉质钙化和显微硬度较低。电子探针分析（EPMA）显示，cKO小鼠牙釉质中钙、磷含量低于对照组。cKO小鼠的成釉细胞层在成熟阶段表现出成釉细胞发育不良。Trpm 7基因敲低的大鼠SF 2细胞出现形态学缺陷。与假转染细胞相比，Trpm 7敲除细胞系显示出较低水平的钙化，茜素红染色阳性，细胞间粘附结构受损。这些结果表明，TRPM 7是一个关键的离子通道，在釉质钙化的成釉细胞在成釉细胞的有效形态发生。
Transient receptor potential melastatin 7 (TRPM7) is a unique ion channel connected to a kinase domain. We previously demonstrated thatTrpm7expression is high in mouse ameloblasts and odontoblasts, and that amelogenesis is impaired in TRPM7 kinase‐dead mice. Here, we analyzed TRPM7 function during amelogenesis inKeratin 14‐Cre;Trpm7fl/flconditional knockout (cKO) mice andTrpm7knockdown cell lines. cKO mice showed lesser tooth pigmentation than control mice and broken incisor tips. Enamel calcification and microhardness were lower in cKO mice. Electron probe microanalysis (EPMA) showed that the calcium and phosphorus contents in the enamel were lower in cKO mouse than in control mice. The ameloblast layer in cKO mice showed ameloblast dysplasia at the maturation stage. The morphological defects were observed in rat SF2 cells withTrpm7knockdown. Compared with mock transfectants, theTrpm7knockdown cell lines showed lower levels of calcification with Alizarin Red‐positive staining and an impaired intercellular adhesion structures. These findings suggest that TRPM7 is a critical ion channel in enamel calcification for the effective morphogenesis of ameloblasts during amelogenesis.
影响因子：
9.8
作者：
Parry, David A.;Poulter, James A.;Mighell, Alan J.
通讯作者：
Mighell, Alan J.
间充质细胞 TRPM7 表达是通过调节软骨形成来形成骨所必需的。
影响因子：
4.1
作者：
Shin M;Mori S;Mizoguchi T;Arai A;Kajiya H;Okamoto F;Bartlett JD;Matsushita M;Udagawa N;Okabe K
通讯作者：
Okabe K
影响因子：
4
作者：
Bomfim, Guilherme H. Souza;Costiniti, Veronica;Lacruz, Rodrigo S.
通讯作者：
Lacruz, Rodrigo S.