A new temperature-insensitive allele of the Arabidopsis AXR6/CUL1 locus derived from a missense mutation in the C-terminal RBX1 binding region
A new temperature-insensitive allele of the Arabidopsis AXR6/CUL1 locus derived from a missense mutation in the C-terminal RBX1 binding region
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拟南芥 AXR6/CUL1 基因座的一个新的温度不敏感等位基因源自 C 端 RBX1 结合区的错义突变
DOI：
10.1080/15592324.2015.1078956
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
K. T.
中科院分区：
文献类型：
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作者：
Mori;Y.;Hayashi;M.;Nishimura;M. and Yamamoto;K. T.
我们从landsbergerecta生态型拟南芥（arabidopsis thaliana）的ems突变群体中分离到一个新的生长素抗性6/CULLIN1（AXR6/CUL1）位点的隐性等位基因AXR6 - 101。axr6 - 101具有生长素抗性，与其他隐性axr6突变体相似，呈半矮化。axr6 - 101表型是由CUL1蛋白的E716K取代引起的，这可能会影响其与RING-box 1 （RBX1）的c端RING结构域的结合能力。先前报道的AXR6的等位基因cul1-7是由T510上的一个取代引起的，该取代与RBX1的n端β链结合。虽然cul1 - 7表现出温度敏感表型，但axr6 - 101的表型在很大程度上不受温度的影响。axr6 - 101可能为研究CUL1蛋白的结构-功能关系提供重要的遗传资源。
We isolated a new recessive allele at theAUXIN RESISTANT6/CULLIN1(AXR6/CUL1) locus,axr6–101, from an EMS-mutagenized population ofArabidopsis thaliana, the Landsbergerectaecotype.axr6–101is auxin resistant and semi-dwarf similar to the other recessiveaxr6mutants. Theaxr6–101phenotype is caused by the E716K substitution of the CUL1 protein, which is likely to affect its ability to bind to the C-terminal RING domain of RING-box 1 (RBX1). The previously reported allele of AXR6,cul1–7, is caused by a substitution at T510 that binds to the N-terminal β-strand of RBX1. Althoughcul1–7shows temperature-sensitive phenotype, theaxr6–101phenotype is largely unaffected by temperature.axr6–101may provide an important genetic resource for study of the structure−function relationship of the CUL1 protein.