Genome Structure of Caulobacter Phage phiCb5
Genome Structure of Caulobacter Phage phiCb5
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DOI：
10.1128/jvi.02256-10
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发表时间：
2011-05-01
影响因子：
5.4
通讯作者：
Tars, Kaspars
中科院分区：
文献类型：
--
作者：
Kazaks, Andris;Voronkova, Tatyana;Tars, Kaspars
已测定了柄杆菌噬菌体phiCb 5的全基因组序列，并鉴定和表征了4个开放阅读框（ORF）。至于相关的病毒，ORF编码成熟蛋白、外壳蛋白、复制酶和裂解蛋白，但与其他光滑病毒科成员不同，phiCb 5的裂解蛋白基因在不同的阅读框架中与复制酶完全重叠。phiCb 5的裂解蛋白比远亲的MS 2噬菌体的裂解蛋白长约两倍，并且推测含有两个跨膜螺旋。对所提出的基因组二级结构的分析揭示了一个稳定的5'茎环，类似于其他的cDNA，和一个显著更短的3'非翻译区（UTR）结构，只有三个茎环。
The complete genome sequence of caulobacter phage phiCb5 has been determined, and four open reading frames (ORFs) have been identified and characterized. As for related phages, the ORFs code for maturation, coat, replicase, and lysis proteins, but unlike other Leviviridae members, the lysis protein gene of phiCb5 entirely overlaps with the replicase in a different reading frame. The lysis protein of phiCb5 is about two times longer than that of the distantly related MS2 phage and presumably contains two transmembrane helices. Analysis of the proposed genome secondary structure revealed a stable 5' stem-loop, similar to other phages, and a substantially shorter 3' untranslated region (UTR) structure with only three stem-loops.