Abundant bacteria and fungi attached to airborne particulates in vegetable plastic greenhouses
Abundant bacteria and fungi attached to airborne particulates in vegetable plastic greenhouses
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DOI：
10.1016/j.scitotenv.2022.159507
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发表时间：
2022-10-21
影响因子：
9.8
通讯作者：
Chen, Jianmin
中科院分区：
文献类型：
--
作者：
Nie, Changliang;Geng, Xueyun;Chen, Jianmin
现代蔬菜塑料大棚的兴起，为世界各地提供了越来越多的食用蔬菜。空气中的细菌和真菌给在密闭塑料温室里辛勤劳作的工人带来了更多的暴露机会。采用非培养的定量聚合酶链式反应和高通量测序技术，对中国蔬菜基地山东典型蔬菜样品中的大气颗粒物微生物区系进行了研究。结果表明，VPGS空气细菌平均浓度分别为1.67×10~3个/m~3(PM2.5)和2.38×10~(3)个/m~(3)(PM10)，空气真菌平均浓度分别为1.49×10~2个/m~3(PM2.5)和3.19×10~(2)个/m~(3)(PM10)。VPGS的优势菌为拉氏杆菌、炭疽杆菌、假单胞菌、芽孢杆菌和不动杆菌。葡萄孢霉属、交链孢属、镰刀菌属、孢子球菌属和枝孢霉属是VPGS中常见的真菌属。VPGS中PM10和PM2.5中细菌的CHO_1较高，差异有统计学意义。VPGS的潜在病原菌包括Picketti Raltonia、Lwoffii不动杆菌、炭疽芽孢杆菌、灰霉病菌和球形分支孢子菌。网络分析表明，大气微生物区系与土壤微生物区系密切相关，土壤微生物区系受人类活动的影响。预测的基因功能表明，VPGS中细菌的功能主要涉及代谢、神经退行性疾病和以病原菌-腐殖菌为主的真菌营养模式。这些发现揭示了VPGS中空气中的微生物群，从而可以应用改善空气质量的战略来保障健康和植被。
The proliferation of modern vegetable plastic greenhouses (VPGS) suppliesmore and more vegetables for food all over the world. The airborne bacteria and fungi induce more exposure opportunities for workers toiling in confined plastic greenhouses. Culture-independent approaches by qPCR and high-throughput sequencing technology were used to study the airborne particulates microbiota in typic VPGS in Shandong, a large base of vegetables in China. The result revealed the mean airborne bacteria concentrations reached 1.67 x 103 cells/m(3) (PM2.5) and 2.38 x 10(3) cells/m(3) (PM10), and the mean airborne fungal concentrations achieved 1.49 x 102 cells/m3 (PM2.5) and 3.19 x 10(2) cells/ m(3) (PM10) in VPGS. The predominant bacteria in VPGS included Ralstonia, Alcanivorax, Pseudomonas, Bacillus, and Acinetobacter. Botrytis, Alternaria, Fusarium, Sporobolomyces, and Cladosporium were frequently detected fungal genera in VPGS. A higher Chao1 of bacteria in PM10 was significantly different from PM2.5 in VPGS. The potential pathogens in VPGS include Raltonia picketti, Acinetobacter lwoffii, Bacillus anthracis, Botrytis cinerea, and Cladosporium sphaerospermum. The network analysis indicated that airborne microbiota was associated with soil microbiota which was affected by anthropologic activities. The predicted gene functions revealed that bacterial functionmainly involved metabolism, neurodegenerative diseases, and fungal trophic mode dominated by Pathotroph-Saprotroph in VPGS. These findings unveiled airborne microbiomes in VPGS so that a strategy for improving air quality can be applied to safeguard health and vegetation.