Expression of epiregulin and amphiregulin in the rat ovary.
Expression of epiregulin and amphiregulin in the rat ovary.
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DOI：
10.1677/jme.0.0330281
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发表时间：
2004-08
影响因子：
3.5
通讯作者：
Toshio Sekiguchi;T. Mizutani;Kazuya Yamada;Takashi Kajitani;T. Yazawa;M. Yoshino;Kaoru Miyamoto
中科院分区：
文献类型：
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作者：
Toshio Sekiguchi;T. Mizutani;Kazuya Yamada;Takashi Kajitani;T. Yazawa;M. Yoshino;Kaoru Miyamoto
我们之前报道过，表皮生长因子（EGF）家族生长因子表皮调节蛋白通过妊娠马血清促性腺激素（PMSG）诱导在大鼠卵巢颗粒细胞中表达。在这项研究中，我们报告了 EGF 家族的另一个成员双调蛋白也通过促性腺激素治疗在大鼠卵巢中诱导产生。 Northern印迹分析显示，PMSG处理24小时后诱导上皮调节蛋白和双调蛋白mRNA的表达，但处理后48小时表达量下降。人绒毛膜促性腺激素 (hCG) 的进一步治疗迅速诱导上皮调节蛋白和双调蛋白基因的表达，并在 hCG 治疗后 4 小时达到最大水平。 PMSG 治疗后 6 小时还观察到双调蛋白 mRNA 水平略有增加。原位杂交显示上皮调节蛋白和双调蛋白 mRNA 位于大窦卵泡的颗粒细胞中。这些时空表达模式与环加氧酶-2 (COX-2) 和孕酮受体 (PR) 的时空表达模式相似。在成年骑行大鼠中，上皮调节蛋白和双调蛋白在发情前期的 1800 小时和 2000 小时强烈诱导，与排卵前 LH 峰值一致。原位杂交研究还表明，在发情前期 2000 小时的排卵前卵泡的颗粒细胞中可检测到上皮调节蛋白和双调蛋白 mRNA，其中 COX-2 和 PR 的转录本与上皮调节蛋白和双调蛋白的转录本共定位。这些观察结果表明，EGF 家族成员上皮调节蛋白和双调蛋白可能在循环大鼠的排卵过程以及诱导未成熟大鼠的排卵中发挥作用。
We have previously reported that the epidermal growth factor (EGF) family growth factor, epiregulin, is expressed in rat ovarian granulosa cells by induction with pregnant mare serum gonadotropin (PMSG). In this study, we report that amphiregulin, another member of the EGF family, was also induced in the rat ovary by gonadotropin treatment. Northern blot analysis revealed that PMSG treatment induced the expression of both epiregulin and amphiregulin mRNA after 24 h, but the expression then decreased 48 h after treatment. Further treatment with human chorionic gonadotropin (hCG) rapidly induced the expression of both epiregulin and amphiregulin genes and maximal levels were reached 4 h after hCG treatment. A marginal increase in amphiregulin mRNA levels was also observed 6 h after PMSG treatment. In situ hybridization revealed that epiregulin and amphiregulin mRNAs were localized in the granulosa cells of large antral follicles. These spatio-temporal expression patterns were similar to those of cyclo-oxygenase-2 (COX-2) and progesterone receptor (PR). In adult cycling rats, epiregulin and amphiregulin were strongly induced at 1800 and 2000 h on proestrus coinciding with the preovulatory LH surge. An in situ hybridization study also showed that epiregulin and amphiregulin mRNAs were detectable in the granulosa cells of preovulatory ovarian follicles at 2000 h on proestrus, where transcripts of COX-2 and PR were co-localized with those of epiregulin and amphiregulin. These observations suggested that the EGF family members, epiregulin and amphiregulin, may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats.