Expression of P2X6 receptors in the enteric nervous system of the rat gastrointestinal tract
Expression of P2X6 receptors in the enteric nervous system of the rat gastrointestinal tract
复制标题
大鼠胃肠道肠神经系统P2X6受体的表达
DOI：
10.1007/s00418-009-0659-0
复制
发表时间：
2010-02-01
影响因子：
2.3
通讯作者：
Xiang, Zhenghua
中科院分区：
文献类型：
--
作者：
Yu, Qiang;Zhao, Zhengqing;Xiang, Zhenghua
应用免疫组织化学双标记荧光法研究P2 X（4）和P2 X（6）受体亚单位在大鼠胃肠道的表达。结果表明，P2 X（6）受体广泛表达于粘膜下神经丛和肌间神经丛。在肌间神经丛中，P2 X（6）受体主要表达于类似Dogiel II型神经元的大型神经元中。这些P2 X（6）受体免疫反应（ir）神经元还表达钙结合蛋白28 K，钙视网膜蛋白和神经元核（NeuN），这些蛋白质是内在感觉神经元的标志物。在粘膜下神经丛中，所有的calbindin 28 K、calretinin和NeuN-ir细胞均为P2 X（6）受体免疫反应阳性。P2 X（6）受体不形成同源多聚体，而是与P2 X（2）或P2 X（4）受体形成异源多聚体。P2 X（4）受体在大鼠胃肠道的神经元中不表达，但在巨噬细胞中表达。这些数据表明，P2 X（6）受体主要表达于内源性感觉神经元上，ATP可能通过P2 X（6）受体与P2 X（2）受体的异聚体结合参与调节这些神经元的生理功能。
Expression of P2X(4) and P2X(6) receptor subunits in the gastrointestinal tract of the rat was studied with double-labeling fluorescence immunohistochemistry. The results showed that P2X(6) receptors were expressed widely in the submucosal and myenteric plexuses. In the myenteric plexus, P2X(6) receptors were expressed mainly in large size neurons which resembled Dogiel type II neurons. These P2X(6) receptor-immunoreactive (ir) neurons also expressed calbindin 28K, calretinin and neuronal nuclei (NeuN), proteins that are markers of intrinsic sensory neurons. In the submucosal plexus, all the calbindin 28K, calretinin and NeuN-ir cells were immunoreactive for P2X(6) receptors. P2X(6) receptors do not form homomultimers, but rather heteromultimers with either P2X(2) or P2X(4) receptors. P2X(4) receptors were not expressed in neurons, but were expressed in macrophages of the rat gastrointestinal tract. These data indicate that P2X(6) receptors are mainly expressed on intrinsic sensory neurons and that ATP, via P2X(6) receptors probably in heteromeric combination with P2X(2) receptors, may be involved in regulating the physiological functions of these neurons.