NITRIC-OXIDE AND CYCLIC-GMP FORMATION UPON ELECTRICAL-FIELD STIMULATION CAUSE RELAXATION OF CORPUS CAVERNOSUM SMOOTH-MUSCLE
NITRIC-OXIDE AND CYCLIC-GMP FORMATION UPON ELECTRICAL-FIELD STIMULATION CAUSE RELAXATION OF CORPUS CAVERNOSUM SMOOTH-MUSCLE
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DOI：
10.1016/0006-291x(90)92168-y
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发表时间：
1990-07-31
影响因子：
3.1
通讯作者：
RAJFER, J
中科院分区：
文献类型：
--
作者：
IGNARRO, LJ;BUSH, PA;RAJFER, J
在存在功能性肾上腺素能和胆碱能阻滞的情况下，电场刺激通过未知机制松弛阴茎海绵体平滑肌。我们在这里报告，电场刺激离体条兔阴茎海绵体促进内源性的形成和释放一氧化氮（NO），亚硝酸盐，和环GMP。在存在乙啶和阿托品的情况下，河豚毒素和钾诱导的去极化可消除对电场刺激的体平滑肌舒张反应，N6-硝基-L-精氨酸、NG-氨基-L-精氨酸、氧合血红蛋白和亚甲蓝可显著抑制体平滑肌舒张反应，但吲哚美辛不影响体平滑肌舒张反应。NG-取代的L-精氨酸类似物的抑制作用几乎完全逆转加入过量的L-精氨酸，但不是D-精氨酸。电场刺激引起的体平滑肌松弛伴随着组织中亚硝酸盐和环GMP水平的快速和显着的增加，所有的反应几乎被NG-硝基-L-精氨酸废除。这些观察结果表明，阴茎勃起可能介导的NO产生的非肾上腺素能-非胆碱能神经传递。
In the presence of functional adrenergic and cholinergic blockade, electrical field stimulation relaxes corpus cavernosum smooth muscle by unknown mechanisms. We report here that electrical field stimulation of isolated strips of rabbit corpus cavernosum promotes the endogenous formation and release of nitric oxide (NO), nitrite, and cyclic GMP. Corporal smooth muscle relaxation in response to electrical field stimulation, in the presence of guanethidine and atropine, was abolished by tetrodotoxin and potassium-induced depolarization, and was markedly inhibited by N6-nitro-L-arginine, NG-amino-L-arginine, oxyhemoglobin, and methylene blue, but was unaffected by indomethacin. The inhibitory effects of NG-substituted analogs of L-arginine were nearly completely reversed by addition of excess L-arginine but not D-arginine. Corporal smooth muscle relaxation elicited by electrical field stimulation was accompanied by rapid and marked increases in tissue levels of nitrite and cyclic GMP, and all responses were nearly abolished by NG-nitro-L-arginine. These observations indicate that penile erection may be mediated by NO generated in response to nonadrenergic-noncholinergic neurotransmission.