3D cyclorama for digital unrolling and visualisation of deformed tubes.
3D cyclorama for digital unrolling and visualisation of deformed tubes.
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DOI：
10.1038/s41598-021-93184-x
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发表时间：
2021-07-19
影响因子：
4.6
通讯作者：
Schneider P
中科院分区：
文献类型：
--
作者：
Rossides C;Pender SLF;Schneider P
结肠隐窝是管状腺体，通过称为隐窝分裂的对称分支过程繁殖。在结直肠癌的早期阶段，正常的裂变过程受到干扰，导致不对称的分支或出芽。出芽隐窝的具有挑战性的形状使得难以制备用于常规组织学的石蜡切片，导致结肠横截面的隐窝仅部分可见。为了研究原位和三维（3D）的隐窝出芽，我们采用X射线显微计算机断层扫描成像完整的结肠，和一种新的方法，我们开发的（3D cyclorama）数字展开它们。在这里，我们提出，验证和验证我们的“3D cyclorama”的方法，数字展开变形管的厚度不均匀。它利用静电学原理将管子改革成一系列洋葱状的表面，这些表面被映射到平面全景图上。这使得能够研究在管深度的几个层上延伸的特征，这里通过两个案例研究证明：（i）人类胎盘中的微绒毛和（ii）用于药物递送的3D打印粘合剂膜。我们的3D cyclorama方法可以为广泛的应用提供新的见解，其中数字展开或展平是必要的，包括长骨，牙根和古代卷轴。
Colonic crypts are tubular glands that multiply through a symmetric branching process called crypt fission. During the early stages of colorectal cancer, the normal fission process is disturbed, leading to asymmetrical branching or budding. The challenging shapes of the budding crypts make it difficult to prepare paraffin sections for conventional histology, resulting in colonic cross sections with crypts that are only partially visible. To study crypt budding in situ and in three dimensions (3D), we employ X-ray micro-computed tomography to image intact colons, and a new method we developed (3D cyclorama) to digitally unroll them. Here, we present, verify and validate our ‘3D cyclorama’ method that digitally unrolls deformed tubes of non-uniform thickness. It employs principles from electrostatics to reform the tube into a series of onion-like surfaces, which are mapped onto planar panoramic views. This enables the study of features extending over several layers of the tube’s depth, demonstrated here by two case studies: (i) microvilli in the human placenta and (ii) 3D-printed adhesive films for drug delivery. Our 3D cyclorama method can provide novel insights into a wide spectrum of applications where digital unrolling or flattening is necessary, including long bones, teeth roots and ancient scrolls.
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影响因子：
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通讯作者：
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