Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymer.
Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymer.
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DOI：
10.1016/j.biomaterials.2006.03.020
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发表时间：
2006-08
期刊：
影响因子：
14
通讯作者：
M. Ogiso;N. Minoura;T. Shinbo;T. Shimizu
中科院分区：
文献类型：
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作者：
M. Ogiso;N. Minoura;T. Shinbo;T. Shimizu
为了发展一种简单、廉价的DNA检测方法，制备了一种分子印迹聚合物（MIP）凝胶电泳（MIPGE），用于识别特异性双链DNA（dsDNA）靶序列。在MIPGE过程中，双链DNA的靶序列的迁移应受到MIP凝胶中结合位点的捕获效应的阻碍。通过绘制MIP凝胶中的迁移距离与凝胶电泳中常用的聚丙烯酰胺凝胶中的迁移距离之间的关系来确定靶dsDNA的这种迁移阻碍。使用该图，实现了从不同大小的dsDNA片段的混合物中检测靶dsDNA。此外，我们发现检测方法成功区分目标和它的碱基对替代品。这些结果表明，MIPGE可用于检测目标dsDNA序列。
To develop a simple and inexpensive DNA detection method, we prepared a molecularly imprinted polymer (MIP) gel for recognizing a specific double-stranded DNA (dsDNA) target sequence in MIP gel electrophoresis (MIPGE). During MIPGE, migration of the target sequence of dsDNA should be hindered by the capture effect of the binding sites in the MIP gel. This migration hindrance of target dsDNA was determined by plotting the relationship between the migration distance in the MIP gel and that in polyacrylamide gel, commonly used in gel electrophoresis. Using this plot, detection of a target dsDNA from a mixture of different-sized dsDNA fragments was achieved. Moreover, we found the detection method successfully distinguished between a target and its base-pair substitutes. These results suggest that MIPGE could be employed for detection of a target dsDNA sequence.