Improved renal function using adenosine triphosphate-magnesium chloride in preservation of canine kidneys subjected to warm ischemia.
Improved renal function using adenosine triphosphate-magnesium chloride in preservation of canine kidneys subjected to warm ischemia.
复制标题
使用三磷酸腺苷-氯化镁保存遭受热缺血的犬肾可改善肾功能。
DOI：
10.1097/00007890-198103000-00009
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发表时间：
1981
期刊：
影响因子：
6.2
通讯作者：
Baue,AE
中科院分区：
文献类型：
--
作者：
Lytton,B;Vaisbort,VR;Glazier,WB;Chaudry,IH;Baue,AE
先前的研究表明，在肾缺血30至60分钟后给予三磷酸腺苷-氯化镁（ATP-MgCl 2）可改善不同种类动物的急性肾功能衰竭。本研究的目的是确定移植前肾脏保存期间向灌注液中添加ATP-MgCl 2是否可以改善肾功能。将狗肾置于恒温缺血35分钟后，在7℃下脉冲灌注保存24小时。将含有白蛋白的灌注液置于有ATP-MgCl 2和不含ATP-MgCl 2的平衡电解质溶液中。脉搏灌注24小时后，自行移植肾脏，移植后3天测定肾功能。结果表明，经缺血再灌注保存的狗肾在移植后3天发生严重的少尿性肾衰竭。然而，如果在灌注液中加入ATP- mgcl2，这些肾脏显示出明显改善的肾功能和ATP水平。这些结果表明，在灌注液中加入atp - mgcl2可以挽救遭受热缺血发作的肾脏。
Previous studies have shown that adenosine triphosphate-magnesium chloride (ATP-MgCl 2) administered after 30 to 60 min of renal ischemia ameliorated the resulting acute renal failure in different species of animals. The purpose of this study was to determine whether addition of ATP-MgCl 2 to the perfusate during renal preservation, prior to transplantation, might improve renal function. Dog kidneys were subjected to normothermic ischemia for 35 min, after which they were preserved by pulsatile perfusion for 24 hr at 7 C. The perfusate contained albumin in a balanced electrolyte solution with and without ATP-MgCl 2. Following 24 hr of pulsatile perfusion, the kidneys were autotransplanted and renal function was determined 3 days post-transplantation. The results indicated that dog kidneys subjected to ischemia followed by perfusion preservation developed severe oliguric renal failure 3 days after transplantation. However, if ATP-MgCl 2 was added to the perfusate, such kidneys demonstrated markedly improved renal function and ATP levels. These results indicate that kidneys which have been subjected to episodes of warm ischemia could be salvaged by addition of ATP-MgCl 2 to the perfusate.