Scanning electron microscopical observation of an osteoblast/osteoclast co-culture on micropatterned orthopaedic ceramics.
Scanning electron microscopical observation of an osteoblast/osteoclast co-culture on micropatterned orthopaedic ceramics.
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DOI：
10.1177/2041731414552114
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发表时间：
2014
影响因子：
8.2
通讯作者：
Young PS
中科院分区：
文献类型：
--
作者：
Halai M;Ker A;Meek RD;Nadeem D;Sjostrom T;Su B;McNamara LE;Dalby MJ;Young PS
在生物材料工程中，植入物的表面可以影响细胞分化、粘附和对植入物的亲和力。在与植入物接触时，骨髓来源的间充质基质细胞表现出向骨形成成骨细胞的分化，这可以改善骨整合。微图案化的过程已被证明可以改善聚合物中的骨整合，但关于陶瓷的报道很少。本研究的目的是建立骨髓间充质干细胞与破骨细胞祖细胞的共培养，并观察对具有30 µm直径凹坑的微图案化氧化锆增韧氧化铝陶瓷的反应。目的是确定这些小凹是否对骨生成具有特定的生物活性，或者是否具有一般的生物活性，并且还刺激可能导致骨质溶解的破骨细胞生成。我们证明了特定的生物活性的微模式对骨生成，更多的结节形成和较少的破骨细胞相比，平面控制。此外，我们发现巨噬细胞和破骨细胞样细胞不与凹坑相互作用，并在凹坑表面形成较少的全尺寸破骨细胞样细胞。这可能在设计陶瓷骨科植入物时发挥作用。
In biomaterial engineering, the surface of an implant can influence cell differentiation, adhesion and affinity towards the implant. On contact with an implant, bone marrow–derived mesenchymal stromal cells demonstrate differentiation towards bone forming osteoblasts, which can improve osteointegration. The process of micropatterning has been shown to improve osteointegration in polymers, but there are few reports surrounding ceramics. The purpose of this study was to establish a co-culture of bone marrow–derived mesenchymal stromal cells with osteoclast progenitor cells and to observe the response to micropatterned zirconia toughened alumina ceramics with 30 µm diameter pits. The aim was to establish whether the pits were specifically bioactive towards osteogenesis or were generally bioactive and would also stimulate osteoclastogenesis that could potentially lead to osteolysis. We demonstrate specific bioactivity of micropatterns towards osteogenesis, with more nodule formation and less osteoclastogenesis compared to planar controls. In addition, we found that that macrophage and osteoclast-like cells did not interact with the pits and formed fewer full-size osteoclast-like cells on the pitted surfaces. This may have a role when designing ceramic orthopaedic implants.