A simple assay for fluorescent siderophores produced by Pseudomonas species and an efficient isolation of pseudobactin.
A simple assay for fluorescent siderophores produced by Pseudomonas species and an efficient isolation of pseudobactin.
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对假单胞菌属物种产生的荧光铁载体的简单测定以及假杆菌素的有效分离。
DOI：
10.1007/bf00205189
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Gould,SJ
中科院分区：
文献类型：
--
作者：
Nowak-Thompson,B;Gould,SJ
使用 C18 反相 HPLC 结合光电二极管阵列检测方法检测了荧光假单胞菌 B10 (JL-3133) 的几种铁结合代谢物（铁载体）。该分析使用的挥发性流动相为 90% 20 mmNH4HCO3/10% MeOH，pH 6.5。它已被证明适用于 otherP。用于检测相关代谢物的荧光菌株。分析型 HPLC 条件的直接放大可以有效制备分离假杆菌素（由假杆菌产生的主要铁载体）。荧光B10 (JL-3133)。
Several iron binding metabolites (siderophores) ofPseudomonas fluorescensB10 (JL-3133) have been detected using C18reverse phase HPLC coupled with photodiode array detection methods. This analysis utilized a volatile mobile phase of 90% 20 mmNH4HCO3/10% MeOH, pH 6.5. It has been shown to be applicable to otherP. fluorescensstrains for the detection of related metabolites. Direct scale-up of the analytical HPLC conditions allowed for the efficient preparative isolation of pseudobactin, the principle siderophore produced byP. fluorescensB10 (JL-3133).