Immunohistochemical analysis of GFAP-δ and nestin in cerebral astrocytomas
Immunohistochemical analysis of GFAP-δ and nestin in cerebral astrocytomas
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DOI：
10.1007/s10014-014-0199-8
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发表时间：
2015-04-01
影响因子：
3.3
通讯作者：
Gorgan, Mircea Radu
中科院分区：
文献类型：
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作者：
Brehar, Felix Mircea;Arsene, Dorel;Gorgan, Mircea Radu
GFAP-δ是胶质细胞酸性蛋白的δ同种型，特别在脑的脑室下区（SVZ）中表达。SVZ中的GFAP-δ阳性细胞共表达神经干细胞（NSC）标记物巢蛋白。根据胶质瘤发生的理论，位于SVZ的具有干细胞特征的细胞群的转化可能是星形细胞瘤的起源。此外，已知癌症干细胞促进脑星形细胞瘤中的肿瘤侵袭。因此，我们研究了GFAP-δ和巢蛋白在脑星形细胞瘤中的免疫染色，并评估了这些标志物的阳性细胞比率与44例II、III和IV级脑星形细胞瘤（世界卫生组织分类）中与肿瘤侵袭相关的神经影像学特征之间的相关性。所有病例均通过立体定向活检获得组织样本。根据术前神经影像学标准，将肿瘤分为高侵袭性和低侵袭性。大多数低级别和高级别星形细胞瘤表达GFAP-δ和巢蛋白。高侵袭性肿瘤中GFAP-δ和nestin的阳性细胞率显著高于低侵袭性肿瘤（p < 0.05）。总之，这些结果表明，GFAP-δ和巢蛋白可能是脑星形细胞瘤中与肿瘤侵袭性相关的临床相关标志物。
GFAP-delta, the delta isoform of the glial fibrillary acidic protein, is particularly expressed in the subventricular zone (SVZ) of the brain. GFAP-delta positive cells in the SVZ co-express the neural stem cells (NSCs) marker nestin. According to the theory of glioma oncogenesis, transformation of a cell population with stem features which resides in the SVZ could be the origin of astrocytomas. Moreover, it is known that cancer stem cells promote tumor invasion in cerebral astrocytomas. Therefore, we investigated the immunostaining of GFAP-delta and nestin in cerebral astrocytomas and evaluated the correlation between the positive cell ratio of these markers and the neuroimaging features associated with tumor invasion in forty-four cases of grade II, III and IV cerebral astrocytomas (World Health Organization's classification). Tissue samples were obtained by stereotactic biopsies in all cases. According to the preoperative neuroimaging criteria, tumors were categorized into highly invasive and low invasive. Most of the low-grade and high-grade astrocytomas express GFAP-delta and nestin. The positive cell ratio of GFAP-delta and the positive cell ratio of nestin were statistically significantly higher in highly invasive tumors compared with low-invasive tumors (p < 0.05). Altogether, these results suggest that GFAP-delta and nestin could be clinically relevant markers associated with tumor invasiveness in cerebral astrocytomas.