Isolation and characterization of novel giant Stenotrophomonas maltophilia phage φSMA5
Isolation and characterization of novel giant Stenotrophomonas maltophilia phage φSMA5
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DOI：
10.1128/aem.71.3.1387-1393.2005
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发表时间：
2005-03-01
影响因子：
4.4
通讯作者：
Weng, SF
中科院分区：
文献类型：
--
作者：
Chang, HC;Chen, CR;Weng, SF
嗜麦芽窄食单胞菌是引起医院感染的最常见的条件致病菌之一。由于大多数分离株对多种抗生素具有耐药性，因此噬菌体治疗的发展引起了人们的强烈关注。在本研究中，8个S.嗜麦芽窄食单胞菌分离自临床样品，包括患者样本、导管相关装置和废水。根据噬菌体DNA在不同限制性内切酶作用下的宿主范围和消化性，这些噬菌体可分为四类。其中之一，命名为φ SMA 5，进一步表征。电子显微镜显示它类似于肌尾病毒科，具有等长的头部（直径90 nm），尾部（90 nm长），基板（25 nm宽）和短尾纤维。的phi SMA 5双链DNA，难消化的大多数限制性内切酶，进行了测试，并估计为250 kb的脉冲场凝胶电泳。该基因组大小仅次于铜绿假单胞菌的最大噬菌体phi KZ。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中，25个病毒粒子蛋白被可视化。其中四个的N-末端测序表明，它们中的每一个都可能有其N-末端切除。在87个S。在本研究中收集的嗜麦芽窄食单胞菌菌株中，只有61株对phi SMA 5敏感，表明噬菌体治疗策略需要更多的药物。由于文献检索未获得关于S.嗜麦芽窄食单胞菌SMA 5似乎是首次报道。
Stenotrophomonas maltophilia is one of the most prevalent opportunistic bacteria causing nosocomial infections. It has become problematic because most of the isolates are resistant to multiple antibiotics, and therefore, development of phage therapy has attracted strong attention. In this study, eight S. maltophilia phages were isolated from clinical samples including patient specimens, catheter-related devices, and wastewater. These phages can be divided into four distinct groups based on host range and digestibility of the phage DNAs with different restriction endonucleases. One of them, designated phi SMA5, was further characterized. Electron microscopy showed it resembled Myoviridae, with an isometric head (90 nm in diameter), a tail (90 nm long), a baseplate (25 run wide), and short tail fibers. The phi SMA5 double-stranded DNA, refractory to digestion by most restriction enzymes, was tested and estimated to be 250 kb by pulsed-field gel electrophoresis. This genome size is second to that of the largest phage, phi KZ of Pseudomonas aeruginosa. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 25 virion proteins were visualized. N-terminal sequencing of four of them suggested that each of them might have had its N terminus cleaved off. Among the 87 S. maltophilia strains collected in this study, only 61 were susceptible to phi SMA5, indicating that more phages are needed toward a phage therapy strategy. Since literature search yielded no information about S. maltophilia phages, phi SMA5 appears to be the first reported.