Mass spectrometry imaging reveals local metabolic changes in skeletal muscle due to chronic training.
Mass spectrometry imaging reveals local metabolic changes in skeletal muscle due to chronic training.
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质谱成像揭示了长期训练导致的骨骼肌局部代谢变化。
DOI：
10.1093/bbb/zbac037
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Fujii NL.
中科院分区：
文献类型：
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作者：
Goto-Inoue N;Morisasa M;Kimura K;Mori T;Furuichi Y;Manabe Y;Fujii NL.
肌肉萎缩是一个重大的健康问题，需要有效的预防和治疗方法。长期运动是治疗萎缩的有效策略，可促进肌肉肥大，从而导致动态代谢变化；然而，不同肌纤维类型的代谢变化有所不同。为了研究长期运动引起的局部代谢变化，我们利用了全面的蛋白质组和质谱 (MS) 成像分析。我们的训练模型仅在糖酵解肌纤维中表现出肥大特征。蛋白质组分析表明，运动促进合成代谢途径，例如蛋白质合成，以及脂质代谢的显着变化，但不会促进葡萄糖代谢。此外，基本能量来源、糖原、中性脂质和 ATP 对运动敏感，并且这些来源的变化在糖酵解肌纤维和氧化肌纤维之间存在差异。 MS 成像显示肌纤维之间的脂质成分不同；花生四烯酸可能是氧化肌纤维肌肉肥大期间促进脂质代谢的有效靶标。
Muscle atrophy is a major health problem that needs effective prevention and treatment approaches. Chronic exercise, an effective treatment strategy for atrophy, promotes muscle hypertrophy, which leads to dynamic metabolic changes; however, the metabolic changes vary among myofiber types. To investigate local metabolic changes due to chronic exercise, we utilized comprehensive proteome and mass spectrometry (MS) imaging analyses. Our training model exhibited hypertrophic features only in glycolytic myofibers. The proteome analyses demonstrated that exercise promoted anabolic pathways, such as protein synthesis, and significant changes in lipid metabolism, but not in glucose metabolism. Furthermore, the fundamental energy sources, glycogen, neutral lipids, and ATP, were sensitive to exercise, and the changes in these sources differed between glycolytic and oxidative myofibers. MS imaging revealed that the lipid composition differs among myofibers; arachidonic acid might be an effective target for promoting lipid metabolism during muscle hypertrophy in oxidative myofibers.