Identification and characterization of microRNA from chicken adipose tissue and skeletal muscle
Identification and characterization of microRNA from chicken adipose tissue and skeletal muscle
复制标题
鸡脂肪组织和骨骼肌中 microRNA 的鉴定和表征
DOI：
10.3382/ps.2011-01656
复制
发表时间：
2012-01-01
期刊：
影响因子：
4.4
通讯作者：
Gu, Z. L.
中科院分区：
文献类型：
--
作者：
Wang, X. G.;Yu, J. F.;Gu, Z. L.
MicroRNA（miRNA）是一类在生物体中广泛表达的非编码小RNA。不同类型在各种生物过程中发挥重要作用，如生长和发育。在本研究中，我们在鸡脂肪组织和骨骼肌中鉴定了47种miRNA，其中38种是已知的鸡miRNA，4种是已知的与其他物种同源的miRNA，5种是潜在的新miRNA。预测了脂肪组织和骨骼肌中的靶基因。表达分析表明，10种选择的miRNA在不同发育阶段差异表达。miRNA-133 a和miR-1a均与肌肉相关，而miR-122与脂肪相关。某些已鉴定的miRNA可能对鸡脂肪组织和骨骼肌的生长和发育至关重要。对这些miRNA的进一步研究将有助于了解它们在家禽脂肪组织和骨骼肌生长发育中的作用。
MicroRNA (miRNA) is small noncoding RNA that is extensively expressed in organisms. Different types play important roles in various biological processes, such as growth and development. In this study, we identified 47 miRNA in chicken adipose tissue and skeletal muscle, of which 38 were known chicken miRNA, 4 were known miRNA homologous to other species, and 5 were potentially novel miRNA. The target genes from adipose tissue and skeletal muscle were predicted. The expression assay indicated that the 10 selected miRNA were differentially expressed in different developmental stages. Both miRNA-133a and miR-1a were muscle-related, whereas miR-122 was adipose-related miRNA. Certain identified miRNA may be essential to growth and development of chicken adipose tissue and skeletal muscle. Further studies of these miRNA will help to understand their functions in growth and development of adipose tissue and skeletal muscle of poultry.