Involvement of N-linked carbohydrate chains of pig zona pellucida in sperm-egg binding.
Involvement of N-linked carbohydrate chains of pig zona pellucida in sperm-egg binding.
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猪透明带的 N 连接碳水化合物链参与精卵结合。
DOI：
10.1111/j.1432-1033.1995.035_1.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
M. Nakano
中科院分区：
文献类型：
--
作者：
N. Yonezawa;H. Aoki;Y. Hatanaka;M. Nakano
猪卵清蛋白的精子受体活性先前已显示存在于其中一种组分中，即猪卵清蛋白3 α（PZP 3 α），其可在通过内切-β-半乳糖苷酶消化去除唾液酸化和/或硫酸化N-乙酰聚（乳糖胺）后纯化。在这项研究中，我们研究了是否N-连接或O-连接的糖链参与精子受体活动的猪透明体。在体外竞争试验中，通过用N-聚糖酶消化，从内切-β-半乳糖苷酶消化的PZP 3 α中消除N-连接的碳水化合物链显著降低了其对精卵结合的抑制作用，而通过碱处理消除O-连接的碳水化合物链几乎没有降低抑制作用。这些结果表明PZP 3 α的N-连接的糖链在介导猪透明带精子结合中起主要作用。
The sperm receptor activity of pig zona pellucida has been previously shown to exist in one of the components, pig zona protein 3 alpha (PZP3 alpha), that can be purified after the removal of sialylated and/or sulfated N-acetylpoly(lactosamine) by digestion with endo-beta-galactosidase. In this study, we examined whether N-linked or O-linked carbohydrate chains are involved in the sperm receptor activity of pig zona pellucida. The elimination of N-linked carbohydrate chains from endo-beta-galactosidase-digested PZP3 alpha by digestion with N-glycanase markedly reduced its inhibitory effect on sperm-egg binding in an in vitro competition assay, whereas the elimination of O-linked carbohydrate chains by alkali treatment hardly reduced the inhibitory effect. These results indicate that N-linked carbohydrate chains of PZP3 alpha play a major role in mediating the sperm binding of zona pellucida in pig.