THE CUTICULAR PROTEINS FROM FREE-LIVING AND PARASITIC STAGES OF HAEMONCHUS-CONTORTUS .1. ISOLATION AND PARTIAL CHARACTERIZATION
THE CUTICULAR PROTEINS FROM FREE-LIVING AND PARASITIC STAGES OF HAEMONCHUS-CONTORTUS .1. ISOLATION AND PARTIAL CHARACTERIZATION
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DOI：
10.1016/0305-0491(89)90360-x
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发表时间：
1989-01-01
影响因子：
2.2
通讯作者：
FETTERER, RH
中科院分区：
文献类型：
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作者：
FETTERER, RH
1.通过机械破碎和清洁剂处理相结合的方法，从成年雄性、成年雌性、感染性幼虫的第二次蜕皮（2 M）鞘（L3（2 M））以及绵羊寄生虫捻转血矛线虫的寄生第三期（L3）中分离角质层。2. 2 ME可溶性蛋白质主要有4 ~ 5条蛋白带，分子量范围为100 ~ 56 kD，最大的分子量为56 kD。雌性成虫的表皮蛋白与雄性相似。3.从幼虫阶段，2 M角质层，和L3角质层，角质层蛋白质，从成人和彼此不同。最突出的蛋白质条带观察到分子量为78和39 kD的L3角质层和100，91和46 kD的2 M角质层。所有发育阶段的2 ME可溶性表皮蛋白至少部分被细菌胶原酶消化。4.角质层蛋白的氨基酸组成是相似的L3和2 M，但成人有较少的甘氨酸和大量的碱性氨基酸比幼虫阶段。与L3（64%）和2 M（22%）相比，2 ME处理溶解的分离角质层的量在成人中最大（80%）。5.这些结果支持了一个假设，即存在定量和定性阶段特异性差异的角质层蛋白的H。扭曲
1. Cuticles were isolated from the adult males, adult females, the second molt (2M) sheath from the infective larvae (L3(2M)), and the parasitic third stage (L3) of the sheep parasite Haemonchus contortus by a combination of mechanical disruption and detergent treatment. 2. The 2ME soluble cuticular proteins from adult males contained 4 or 5 major protein bands with molecular weights ranging from 100 to 56 kD with the most prominent band at 56 kD. The cuticular proteins from adult females were similar to the male. 3. Cuticular proteins from the larval stages, 2M cuticle, and L3 cuticle, differed from the adults and from each other. The most prominent protein bands were observed with molecular weights on 78 and 39 kD for the L3 cuticle and 100, 91 and 46 kD for the 2M cuticle. The 2ME soluble cuticular proteins from all developmental stages were at least partially digested by bacterial collagenase. 4. The amino acid composition of cuticular proteins was similar for the L3 and 2M, but adults had lesser amounts of glycine and greater amounts of basic amino acids than the larval stages. The amount of the isolated cuticle solubilized by the 2ME treatment was greatest in adults (80%) compared to the L3 (64%) and the 2M (22%). 5. These results support a hypothesis that there are quantitative and qualitative stage specific differences in the cuticular proteins of H. contortus.