Nucleic acid-mediated intracellular protein delivery by lipid-like nanoparticles.
Nucleic acid-mediated intracellular protein delivery by lipid-like nanoparticles.
复制标题
核酸介导的细胞内蛋白通过脂质样纳米颗粒递送。
DOI：
10.1016/j.biomaterials.2014.04.014
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发表时间：
2014-08
期刊：
影响因子：
14
通讯作者：
Anderson DG
中科院分区：
文献类型：
--
作者：
Eltoukhy AA;Chen D;Veiseh O;Pelet JM;Yin H;Dong Y;Anderson DG
Intracellular protein delivery has potential biotechnological and therapeutic application, but remains technically challenging. In contrast, a plethora of nucleic acid carriers have been developed, with lipid-based nanoparticles (LNPs) among the most clinically advanced reagents for oligonucleotide delivery. Here, we validate the hypothesis that oligonucleotides can serve as packaging materials to facilitate protein entrapment within and intracellular delivery by LNPs. Using two distinct model proteins, horseradish peroxidase and NeutrAvidin, we demonstrate that LNPs can yield efficient intracellular protein delivery in vitro when one or more oligonucleotides have been conjugated to the protein cargo. Moreover, in experiments with NeutrAvidin in vivo, we show that oligonucleotide conjugation significantly enhances LNP-mediated protein uptake within various spleen cell populations, suggesting that this approach may be particularly suitable for improved delivery of protein-based vaccines to antigen-presenting cells.
