Production of autoantibodies by human-human hybridomas.
Production of autoantibodies by human-human hybridomas.
复制标题
人-人杂交瘤产生自身抗体。
DOI：
10.1172/jci110595
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Schwartz,RS
中科院分区：
文献类型：
--
作者：
Shoenfeld,Y;Hsu-Lin,SC;Gabriels,JE;Silberstein,LE;Furie,BC;Furie,B;Stollar,BD;Schwartz,RS
利用自身免疫性疾病患者外周血淋巴细胞和脾细胞制备产生自身抗体的人-人杂交瘤。由于未使用外源免疫，杂交瘤抗体来源于自发产生自身抗体的B细胞。混合动力车从4254辆增加到108辆（2.5%）。获得GM 4672骨髓瘤系杂交瘤的最佳条件为：在2 ml的孔中初始生长，使用44%的聚乙二醇，单个核细胞/GM 4672细胞比例为5:1，预先用美洲商麻有丝分裂原刺激B淋巴细胞。杂交瘤上清液对ssDNA、血小板和红细胞有活性。结果表明，从各种自身免疫性疾病患者的淋巴细胞中产生人-人杂交瘤是可行的。
Peripheral blood lymphocytes and splenocytes of patients with autoimmune disease were used to prepare human-human hybridomas that produce autoantibodies. Because exogenous immunization was not used, the hybridoma antibodies were derived from B cells that spontaneously produced autoantibodies. 108 hybrids grew from 4,254 wells (2.5%). Optimal conditions for obtaining hybridomas with the GM 4672 myeloma line included initial growth in 2-ml wells, the use of 44% polyethylene glycol, a mononuclear cell/GM 4672 cell ratio 5:1, and prior stimulation of the B lymphocytes with pokeweed mitogen. Hybridoma supernatants had activity against ssDNA, platelets, and erythrocytes. The results demonstrate the feasibility of producing human-human hybridomas from lymphocytes of patients with various autoimmune diseases.