Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein-Protein Binding Affinities
Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein-Protein Binding Affinities
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DOI：
10.1021/nl501865p
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发表时间：
2014-10-01
期刊：
影响因子：
10.8
通讯作者：
Soennichsen, Carsten
中科院分区：
文献类型：
--
作者：
Ahijado-Guzman, Ruben;Prasad, Janak;Soennichsen, Carsten
目前大多数用于定量蛋白质-蛋白质相互作用的技术需要一次分析一对结合伴侣。在这里，我们提出了一种无标记，简单，快速，具有成本效益的路线，同时表征多个大分子伴侣之间的结合亲和力，使用光学暗场光谱和单个蛋白质功能化的金纳米棒作为传感元件。我们的NanoSPR方法可以很容易地成为生物，生物化学和医学实验室的简单和标准工具。
Most of current techniques used for the quantification of protein-protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.