A simple and rapid method for the preparation of plasma membranes.
A simple and rapid method for the preparation of plasma membranes.
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一种简单、快速的质膜制备方法。
DOI:
10.1016/0005-2736(83)90404-2
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发表时间:
1983
期刊:
影响因子:
--
通讯作者:
Mehdi,SQ
中科院分区:
文献类型:
--
作者:
Maeda,T;Balakrishnan,K;Mehdi,SQ
A simple and rapid method for preparing plasma membranes from isolated cells or tissues is described. The membranes were characterised (a) biochemically by an analysis of specific marker enzymes, (b) by quantitation of cell surface receptors, and (c) immunologically by their ability to elicit specific allogeneic responses from cytotoxic T cells in secondary in vitro stimulations. Based on both biochemical and immunologic criteria, plasma membranes prepared by the method described here are of equal or greater ‘purity’ compared to those prepared by two other methods that are most widely used to date and the yields are several-fold higher.
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影响因子:
20.3
作者:
Ramsay,N;LeBien,T;Nesbit,M;McGlave,P;Weisdorf,D;Kenyon,P;Hurd,D;Goldman,A;Kim,T;Kersey,J
通讯作者:
Kersey,J
影响因子:
11.2
作者:
Dewey,WC
通讯作者:
Dewey,WC
DOI:
--
发表时间:
1985
期刊:
Journal of immunology (Baltimore, Md. : 1950)
影响因子:
--
作者:
Uckun,FM;Stong,RC;Youle,RJ;Vallera,DA
通讯作者:
Vallera,DA
DOI:
--
发表时间:
1983
期刊:
影响因子:
--
作者:
E. Thomas
通讯作者:
E. Thomas
DOI:
10.1200/jco.1984.2.9.1050
发表时间:
1984
期刊:
Journal of clinical oncology : official journal of the American Society of Clinical Oncology
影响因子:
--
作者:
Robins,HI;Dennis,WH;Steeves,RA;Sondel,PM
通讯作者:
Sondel,PM