miR‐17‐5p promotes proliferation by targeting SOCS6 in gastric cancer cells
miR‐17‐5p promotes proliferation by targeting SOCS6 in gastric cancer cells
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DOI：
10.1016/j.febslet.2014.04.036
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发表时间：
2014-06
期刊：
影响因子：
3.5
通讯作者：
Qiong Wu;G. Luo;Zhiping Yang;Fei Zhu;Y. An;Yongquan Shi;D. Fan
中科院分区：
文献类型：
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作者：
Qiong Wu;G. Luo;Zhiping Yang;Fei Zhu;Y. An;Yongquan Shi;D. Fan
本研究旨在探讨miR-17- 5 p在胃癌中的确切功能及其可能机制。实时荧光定量PCR检测发现miR-17- 5 p在胃癌组织中的表达高于正常组织。功能获得和功能丧失测定表明，miR-17- 5 p在体外和体内增加胃癌细胞的增殖和生长。通过报告基因和蛋白质印迹分析，SOCS 6被证明是miR-17- 5 p的直接靶点，并且增殖实验证实SOCS 6在胃癌中发挥与miR-17- 5 p相反的功能。总之，miR-17- 5 p可能通过抑制SOCS 6在胃癌中发挥促增殖因子的作用。
This study aimed to test the exact functions and potential mechanisms of miR-17-5p in gastric cancer. Using real-time PCR, miR-17-5p was found to be expressed more highly in gastric cancer compared with-normal tissues. Gain- and loss-of-function assays demonstrated that miR-17-5p increased the proliferation and growth of gastric cancer cells in vitro and in vivo. Through reporter gene and western blot assays, SOCS6 was shown to be a direct target of miR-17-5p, and proliferative assays confirmed that SOCS6 exerted opposing function to that of miR-17-5p in gastric cancer. In short, miR-17-5p might function as a pro-proliferative factor by repressing SOCS6 in gastric cancer.