Synthesis, photodynamic activities, and cytotoxicity of new water-soluble cationic gallium(III) and zinc(II) phthalocyanines
Synthesis, photodynamic activities, and cytotoxicity of new water-soluble cationic gallium(III) and zinc(II) phthalocyanines
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DOI：
10.1016/j.jinorgbio.2018.11.013
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发表时间：
2019-03-01
影响因子：
3.9
通讯作者：
Ikeue, Takahisa
中科院分区：
文献类型：
--
作者：
Fujishiro, Rei;Sonoyama, Hayato;Ikeue, Takahisa
合成了8个β-位含N-甲基吡啶基团的阳离子镓(III)和锌(II)酞菁。这些配合物在二甲基亚砜(DMSO)中高度溶解，在水和磷酸盐缓冲盐水(PBS)中中等溶解，在1.2×10~(-4)M和1.5×10~(-5)M的水中均不聚集。与锌(II)络合物相比，Ga(III)Cl络合物具有较高的水溶性，这归因于轴向配位氯化物的存在。研究了这些配合物的光谱性质、单线态氧(O-1(2))的光生成和细胞毒性。在DMSO溶液中，用Ga(III)Cl和Zn(II)络合物光生单线态氧的绝对量子产率(Phi Delta)分别为4.4%和5.3%。在人HEp2细胞中评价了镓(III)、氯和锌(II)配合物的细胞毒性和细胞内定位。这两种配合物都定位在细胞内的多个细胞器中，在黑暗中没有显示出细胞毒性。然而，在低光剂量(1.5J/cm(2))下，锌(II)络合物表现出较高的光细胞毒性。结果表明，锌(II)络合物可作为光动力疗法(PDT)的潜在光敏剂。
The cationic Ga(III) and Zn(II) phthalocyanines carrying N-methyl-pyridinium groups at eight peripheral beta-positionshave been synthesized. These complexes are highly soluble in dimethyl sulfoxide (DMSO) and moderately soluble in water and phosphate buffered saline (PBS); both Ga(III)Cl and Zn(II) complexes have shown no aggregation in water up to 1.2 x 10(-4) and 1.5 x 10(-5) M, respectively. A higher water-solubility of Ga(III)Cl complex as compared to Zn(II) complex is ascribed to the presence of an axially coordinated chloride. The spectroscopic properties, photogeneration of singlet oxygen (O-1(2)), and cytotoxicity of these complexes have been investigated. The absolute quantum yields (Phi Delta(absolute)) for the photogeneration of singlet oxygen using Ga(III)Cl and Zn(II) complexes have been determined to be 4.4 and 5.3%, respectively, in DMSO solution. The cytotoxicity and intracellular sites of localization of Ga(III)Cl and Zn(II) complexes have been evaluated in human HEp2 cells. Both complexes, localized intracellularly in multiple organelles, have shown no cytotoxicity in the dark. Upon exposure to a low light dose (1.5 J/cm(2)), however, Zn(II) complex has exhibited a high photocytotoxicity. The result suggests that Zn(II) complex can be considered as a potential photosensitizer for Photodynamic therapy (PDT).