NUTRITIONAL STUDIES OF RICKETTSIA-QUINTANA - NATURE OF HEMATIN REQUIREMENT
NUTRITIONAL STUDIES OF RICKETTSIA-QUINTANA - NATURE OF HEMATIN REQUIREMENT
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DOI：
10.1128/jb.109.1.89-95.1972
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发表时间：
1972-01-01
影响因子：
3.2
通讯作者：
OSTERMAN, JV
中科院分区：
文献类型：
--
作者：
MYERS, WF;WISSEMAN, CL;OSTERMAN, JV
在由脑心浸液基质补充淀粉和血红素组成的液体培养基中的五他那格雷立克次体。对血红素的生长需要不能被已知对H2 O2具有催化活性的化合物，即，过氧化氢酶、丙酮酸钾或木炭，或还原化合物亚硫酸钠和巯基乙酸钠。quintana为过氧化氢酶阴性，但过氧化氢酶-氨基三唑技术不能证明H2 O2的产生。启动生长所需的最小接种物为105个细胞/ml。33 ℃世代时间为10小时，生长温度范围为28 ~ 37 ℃.当添加琥珀酸或谷氨酸作为能量源时，生长得到增强。
Rickettsia quintanagrew in a liquid medium consisting of a brain-heart infusion base supplemented with starch and hematin. The growth requirement for hematin could not be substituted by compounds of known catalytic activity for H2O2, viz., catalase, potassium pyruvate, or charcoal, or by the reducing compounds sodium sulfite and sodium thioglycollate.R. quintanawas catalase-negative, but no H2O2production could be demonstrated by the catalase-aminotriazole technique. A minimum inoculum giving 105cells/ml was required to initiate growth. The generation time at 33 C was 10 hr. The temperature range for growth was 28 to 37 C. Growth was enhanced when succinate or glutamate was added as energy source.