Rapid DNA extraction for molecular epidemiological studies of malaria
Rapid DNA extraction for molecular epidemiological studies of malaria
复制标题
DOI：
10.1016/s0001-706x(98)00090-4
复制
发表时间：
1999-03-15
期刊：
影响因子：
2.7
通讯作者：
Beck, HP
中科院分区：
文献类型：
--
作者：
Henning, L;Felger, I;Beck, HP
从分子流行病学研究中收集的血液样本中分离 DNA 对于数据的质量和可重复性至关重要。通过四种不同的 DNA 分离方法制备了来自两个疟疾流行地点的血液样本，随后对恶性疟原虫的 msp2 基因座进行 PCR 扩增。我们测试了快速煮沸的方法；异硫氰酸胍DNA提取； QIAmp(TM) 血液试剂盒；和ISOCODE(TM)STIX PCR模板制备试纸，并分析并发感染数/样本。快速沸腾方法和 ISOCODE(TM) STIX 总体上提供了最佳的灵敏度且易于操作。在环境温度下储存和运输 ISOCODE(TM) STIX 的可能性进一步增加了该方法的优势。 (C) 1999 年由 Elsevier Science B.V. 出版。保留所有权利。
DNA isolation from blood samples collected in molecular epidemiological studies is crucial for the qualify and reproducibility of data. Blood samples from two malaria endemic sites have been prepared by four different DNA isolation methods with subsequent PCR amplification of the msp2 locus of Plasmodium falciparum. We tested a rapid boiling method; the guanadine isothiocyanate DNA extraction; QIAmp(TM) blood kit; and the ISOCODE(TM) STIX PCR template preparation dipstick, and analysed the numbers of concurrent infections/sample. The rapid boiling method and the ISOCODE(TM) STIX provided overall the best sensitivity combined with ease of handling. The possibility to store and ship the ISOCODE(TM) STIX at ambient temperature adds further advantage to this method. (C) 1999 Published by Elsevier Science B.V. All rights reserved.