Identification of T lymphocytes in ocular adnexal neoplasms by hybridoma monoclonal antibodies.
Identification of T lymphocytes in ocular adnexal neoplasms by hybridoma monoclonal antibodies.
复制标题
通过杂交瘤单克隆抗体鉴定眼附属器肿瘤中的 T 淋巴细胞。
DOI：
10.1016/0002-9394(83)90019-3
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发表时间：
1983
影响因子：
4.2
通讯作者：
Jakobiec,FA
中科院分区：
文献类型：
--
作者：
Knowles2nd,DM;Jakobiec,FA
为了测定28例眼附件淋巴样肿瘤中辅助T细胞（OKT3+T4+T8−）和抑制/细胞毒性T细胞（OKT3+T4+T8+）的百分比，我们使用OKT系列杂杂瘤单克隆抗体进行间接免疫荧光检测。OKT3+T4+（辅助性）T细胞在10种良性多克隆眼淋巴样增生中均占绝大多数。在这10例中，辅助性t细胞与抑制性t细胞的比值（T4-8）从2.5到8.2不等（平均5.4）。相比之下，24个良性反应性淋巴结的平均T4-T8比值为3.4。这些发现强烈表明，多克隆眼淋巴细胞增生代表了一种T细胞抗原依赖性反应，其特征是辅助性T细胞的增殖，进而驱动B细胞增殖和分化，最终导致临床可检测肿瘤的形成。在18例眼部和16例淋巴结单克隆b细胞增生中，平均T4-T8比值为2.3，表明这些增生中的良性T细胞代表残余细胞群，而不是源于b细胞肿瘤的独特亚群。
We performed indirect immunofluorescence with the OKT series of hybridoma monoclonal antibodies in order to determine the total number of T cells and their subset distribution, that is, the percentages of helper (OKT3+T4+T8−) and suppressor/cytotoxic (OKT3+T4+T8+) T cells, in 28 ocular adnexal lymphoid neoplasms. OKT3+T4+(helper) T cells vastly predominated in each of ten benign, polyclonal ocular lymphoid proliferations. The helper-suppressor T-cell ratio (T4–8) ranged from 2.5 to 8.2 (mean, 5.4) in these ten cases. In comparison, the mean T4–T8 ratio in 24 benign reactive lymph nodes was 3.4. These findings strongly suggested that the polyclonal ocular lymphoid proliferations represent a T-cell antigen-dependent response characterized by a proliferation of helper T cells, which in turn drive B cells to proliferate and to differentiate, eventually resulting in the formation of a clinically detectable tumor. The mean T4-T8 ratio was 2.3 in 18 ocular and in 16 nodal monoclonal B-cell proliferations, suggesting that the benign T cells in these proliferations represent a residual cell population rather than a distinctive subset originating in response to the B-cell neoplasm.