The use of fluorescently-tagged apoptolidins in cellular uptake and response studies.
The use of fluorescently-tagged apoptolidins in cellular uptake and response studies.
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荧光标记的凋亡素在细胞摄取和反应研究中的应用。
DOI：
10.1038/ja.2016.22
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Sulikowski,GaryA
中科院分区：
文献类型：
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作者：
Chong,KatherineM;Leelatian,Nalin;Deguire,SeanM;Brockman,AsaA;Earl,David;Ihrie,RebeccaA;Irish,JonathanM;Bachmann,BrianO;Sulikowski,GaryA
凋亡素是糖大环内酯类微生物代谢产物，据报道对肿瘤细胞具有选择性的细胞毒作用。使用荧光标记的活性衍生物，我们证明了这四个标记的糖大环内酯在癌细胞中的选择性摄取超过了健康的人类血细胞。我们还展示了这五种荧光标记的糖大环内酯在荧光流式细胞术中的应用，以监测细胞对六种糖大环内酯类化合物的摄取和细胞反应。
The apoptolidins are glycomacrolide microbial metabolites reported to be selectively cytotoxic against tumor cells. Using fluorescently tagged active derivatives we demonstrate selective uptake of these four tagged glycomacrolides in cancer cells over healthy human blood cells. We also demonstrate the utility of these five fluorescently tagged glycomacrolides in fluorescent flow cytometry to monitor cellular uptake of the six glycomacrolides and cellular response.