Characterization of distinct Gal:3-O-sulfotransferase activities in human tumor epithelial cell lines and of calf lymph node GlcNAc : 6-O-sulfotransferase activity.
Characterization of distinct Gal:3-O-sulfotransferase activities in human tumor epithelial cell lines and of calf lymph node GlcNAc : 6-O-sulfotransferase activity.
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人肿瘤上皮细胞系中不同 Gal:3-O-磺基转移酶活性和小牛淋巴结 GlcNAc:6-O-磺基转移酶活性的表征。
DOI：
10.1023/a:1007074005371
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发表时间：
1999
影响因子：
3
通讯作者：
Matta,KL
中科院分区：
文献类型：
--
作者：
Chandrasekaran,EV;Jain,RK;Rhodes,JM;Chawda,R;Piskorz,C;Matta,KL
我们早前发现，在人类乳腺和结肠肿瘤中，Gal: 3- o -硫转移酶活性水平的增强分别表明，黏液蛋白核心2结构上的受体优先于血型t -半抗原（A组酶）或galβ 1,4 glcnac （B组酶）[Chandrasekaran EV, Jain RK, Vig R, and Matta KL（1997）糖生物学7:753-68]。本研究报道了这些酶在人肿瘤细胞系和正常组织中的活性。人结肠肿瘤上皮细胞系与其亲本肿瘤相似，表达B组酶活性。结肠癌系LS180酶活性的受体特异性和动力学性质，如二价金属离子活化和pH依赖性活性谱，与结肠组织标本相同。与乳腺肿瘤标本一致，A组酶活性存在于人类乳腺肿瘤上皮细胞系中，有一些例外。Gal: 3- o -磺基转移酶显示出与金萼花凝集素的特异性结合，表明在这些酶上存在含有内核α1,6-聚焦基残基的天冬酰胺连接碳水化合物链。犊牛淋巴结含有GlcNAc: 6- o -硫转移酶和A组Gal: 3- o -硫转移酶活性，它们在pH依赖性、pH最优值（分别为7.6和7.0）和Mn2+的影响方面存在差异。
We found earlier in human breast and colon tumors, an augmented level of Gal : 3-O-sulfotransferase activities showing, respectively, an acceptor preference to blood group T-hapten (Group A enzymes) or Galβ1,4GlcNAc (Group B enzymes) on the mucin Core 2 structure [Chandrasekaran EV, Jain RK, Vig R, and Matta KL (1997) Glycobiology 7: 753–68]. The present study reports these enzyme activities in human tumor cell lines and normal tissues. The human colon tumor epithelial cell lines, akin to their parent tumors, express Group B enzyme activity. The acceptor specificity and kinetic properties, such as divalent metal ion activation and pH dependent activity profile, of the colon cancer line LS180 enzyme activity are identical to those of colon tissue specimens. Consistent with breast tumor specimens, the Group A enzyme activity is present in human breast tumor epithelial cell lines, with some exceptions. The Gal : 3-O-sulfotransferases show specific binding to Aleuria aurantia lectin, suggesting the presence of asparagine linked carbohydrate chains containing an inner core α1,6-fucosyl residue on these enzymes. Calf lymph nodes contain GlcNAc : 6-O-sulfotransferase as well as Group A Gal : 3-O-sulfotransferase activities, which differ in pH dependent profiles, pH optima (7.6 and 7.0, respectively) and the influence of Mn2+.