Screening of adhesive lactobacilli with antagonistic activity against Campylobacter jejuni
Screening of adhesive lactobacilli with antagonistic activity against Campylobacter jejuni
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具有拮抗空肠弯曲菌活性的粘附性乳杆菌的筛选
DOI：
10.1016/j.foodcont.2014.03.042
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发表时间：
2014-10-01
期刊：
影响因子：
6
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Wang, Gang;Zhao, Yu;Chen, Wei
通过对78株乳酸菌（LAB）培养上清液对空肠弯曲菌生长的抑菌圈分析，筛选出15株对空肠弯曲菌具有较强抑菌活性的LAB菌株。其中植物乳杆菌N8、N9、ZL 5和干酪乳杆菌ZL 4对HT-29细胞具有较强的粘附能力。这4株菌的无细胞上清液（CF 5）均含有较高浓度的有机酸，对空肠弯曲菌的抑制作用具有pH敏感性。此外，这4株菌均能强烈拮抗空肠弯曲菌对HT-29细胞的粘附和侵袭，并对人工胃液和人工肠液表现出良好的耐受性。本研究表明，乳杆菌N8、N9、ZL 4和ZL 5菌株可作为潜在的益生菌应用于食品中，以对抗C.空肠感染(C)2014爱思唯尔有限公司版权所有。
In the present study, 78 lactic acid bacteria (LAB) strains were screened for the antagonistic activity against Campylobacter jejuni and 15 LAB strains with high bactericidal capacity were selected for further study through the analysis of the inhibition zone of LAB spent culture supernatant on the growth of C jejuni. Among these strains, four isolates, Lactobacillus plantarum N8, N9, ZL5 and Lactobacillus casei ZL4 exhibited high adhesion ability to HT-29 cells. All the cell free supernatant (CF5) of these four strains contained high concentration of organic acid and their inhibition effects against C jejuni were pH sensitive. Furthermore, these four strains could strongly antagonize the adhesion and invasion of C jejuni to HT-29 cells and showed good tolerance to artificial gastric and small intestinal juices. This study suggests that Lactobacillus strains N8, N9, ZL4 and ZL5 could be used as potential probiotics in food applications against C. jejuni infection. (C) 2014 Elsevier Ltd. All rights reserved.