Force spectroscopy study of the adhesion of plasma proteins to the surface of a dialysis membrane:: Role of the nanoscale surface hydrophobicity and topography
Force spectroscopy study of the adhesion of plasma proteins to the surface of a dialysis membrane:: Role of the nanoscale surface hydrophobicity and topography
复制标题
DOI：
10.1002/jbm.10168
复制
发表时间：
2002-09-05
期刊：
影响因子：
--
通讯作者：
Samorì, B
中科院分区：
文献类型：
--
作者：
Conti, M;Donati, G;Samorì, B
利用扫描力显微镜（SFM）在力谱模式下对血浆蛋白与透析膜表面的粘附过程进行了机械化学研究。将三种具有代表性的血浆蛋白（纤维连接蛋白、纤维蛋白原和白蛋白）共价移植到SFM探针上，并测量这些蛋白对纤维素和合成透析膜的粘附力。为了模拟血液过滤过程中施加在蛋白质分子上的挤压力，该实验对吸附在表面的蛋白质分子施加了可控的负荷。用这种研究蛋白质和透析膜之间相互作用的新方法测量的去黏附力表明，在纳米尺度上，膜的形貌在粘附过程中起着关键作用。在具有与透析膜相同的主要亲水性的扁平玻璃表面上进行的平行实验有力地支持了这一结果。相比之下，疏水性聚苯乙烯表面导致的脱粘力至少要大一个数量级，压倒了蛋白质分子和表面之间任何可能的形状识别过程。(C) 2002 Wiley期刊有限公司
A mechanochemical study of the process of adhesion of plasma proteins to the surface of dialysis membranes was carried out with a scanning force microscope (SFM) in the force spectroscopy mode. Three representative blood plasma proteins (fibronectin, fibrinogen, and albumin) covalently were grafted to a SFM probe, and the adhesion forces of these proteins to cellulosic and synthetic dialysis membranes were measured. The experiment was tailored to apply a controlled load on the protein molecules adsorbed onto the surface in order to simulate the squeezing forces exerted on them during blood filtration. The de-adhesion forces, measured using this new approach for studying the interaction between a protein and dialysis membranes, suggest that the membrane's topography, at a nanometer scale, plays a critical role in the adhesion process. This result was strongly supported by parallel experiments performed on a flattened glass surface with the same dominant hydrophilic character as dialysis membranes. In contrast, a hydrophobic polystyrene surface led to de-adhesion forces at least one order of magnitude greater, overwhelming any possible shape recognition process between the protein molecules and the surface. (C) 2002 Wiley Periodicals, Inc.