Electrochemical and microbial community responses of electrochemically active biofilms to copper ions in bioelectrochemical systems
Electrochemical and microbial community responses of electrochemically active biofilms to copper ions in bioelectrochemical systems
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生物电化学系统中电化学活性生物膜对铜离子的电化学和微生物群落反应
DOI：
10.1016/j.chemosphere.2018.01.009
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发表时间：
2018
期刊：
影响因子：
8.8
通讯作者：
Yuan Yong
中科院分区：
文献类型：
--
作者：
Zhang Yaping;Li Guanqun;Wen Jing;Xu Yangao;Sun Jian;Ning Xun an;Lu Xingwen;Wang Yujie;Yang Zuoyi;Yuan Yong
在生物电化学系统中，重金属对溶液的导电性、发电能力和微生物的活性有着重要的影响。然而，重金属对电化学活性生物膜（EABs）的产电代谢和胞外电子传递过程的影响尚不清楚。在此，我们研究了Cu 2+在逐渐增加的浓度对形态和电化学性能的阳极生物膜和细菌群落的影响，在混合培养的BES。电压输出持续下降，在10 mg L-1时降至零，这归因于毒性抑制，导致阳极生物膜损伤和外膜细胞色素分泌减少。当停止向阳极室引入Cu 2+时，最大产生电压恢复到BES产生电压的75.1%，库仑效率提高，但乙酸盐去除率降低，表明EABs的恢复能力高于非电活性菌。此外，SEM-EDS和XPS表明，大部分Cu 2+被吸附在阳极电极上，并被阳极上的EABs还原。与开路BES相比，通过电路的电子流动可以改善铜的还原。群落分析表明，在阳极室中，土壤细菌对Cu ~（2+）冲击的反应减少，而Stenotrophomasin的反应增加。
Heavy metals play an important role in the conductivity of solution, power generation and activity of microorganisms in bioelectrochemical systems (BESs). However, effect of heavy metal on the process of exoelectrogenesis metabolism and extracellular electron transfer of electrochemically active biofilms (EABs) was poorly understood. Herein, we investigated the impact of Cu2+at gradually increasing concentration on the morphological and electrochemical performance and bacterial communities of anodic biofilms in mixed-culture BESs. The voltage output decreased continuously and dropped to zero at 10 mg L−1, which was attributed to the toxic inhibition that cased anodic biofilm damage and decreased secretion of outer membrane cytochromes. When stopping the introduction of Cu2+to anodic chamber, the maximum voltage production recovered 75.1% of the voltage produced from BES and coulombic efficiency was higher but acetate removal rate was lower than that before Cu2+addition, demonstrating the recovery capability of EABs was higher compared to nonelectroactive bacteria. Moreover, SEM-EDS and XPS suggested that most of Cu2+was adsorbed by the anode electrode and reduced by EABs on anode. Compared to the open-circuit BES, the flow of electrons through a circuit could improve the reduction of copper. Community analysis showed a decrease inGeobacteraccompanied by an increase inStenotrophomonasin response to Cu2+shock in anodic chamber.